CORE GRANT FOR VISION RESEARCH

43名视觉科学家申请资金支持5个研究模块 促进和加强视觉科学家之间的互动 在眼科研究所有不同学科的代表。这些模块 将通过提供以下服务来扩展个人研究计划的努力 为调查人员不具备的项目提供协作机会 专业知识、资金或技术能力。 。这些模块是：形态学、组织培养/单抗、动物 资源、实验室计算机应用和生物统计学。这个 形态模块将提供光学和电子显微镜能力 以及组织化学和原位杂交的组织准备， 从而增加了形态技术的可用性，以 需要相关知识的生物化学家、药理学家和免疫学家 为他们的研究提供了形态数据。组织培养/克隆 抗体模块将帮助PI选择适当的培养技术 和媒体，并在生产眼部起源的特征性组织培养时， 并将负责细胞融合和指定克隆的存储 用于生产单抗。这样的资源将增加 研究人员的主要专长和动物方面的协助 外科技术和术后护理，麻醉管理， 药物和药物，以及抗原注射和出血所需的 多克隆抗体的产生。实验室计算机应用 模块将通过以下方式为个人和协作工作提供支持 协助微型计算机与实验室设备的接口以及 开发用于采集、处理和分析实验的软件 由微型计算机系统提供的数据。生物统计模块将提供 通过协助个人和协作工作来支持这两项工作 临床医生和基础研究人员在研究设计和统计方面的知识 数据分析；主要模块的利用将在规划、设计、 以及临床研究的执行和研究的统计分析 数据。 。这些模块的工作人员都受过专门的 各自的字段。每个模块都在一名 成熟、经验丰富的调查员(S)。模块头部构成了 核心拨款管理委员会，该委员会向 高级调查人员是ERI研究部门的负责人。

Funds are requested by 43 vision scientists to support 5 research modules to facilitate and enhance interaction among the vision scientists in the various disciplines represented at the Eye Research Institute. The modules will extend endeavors of individual research programs by providing collaborative opportunities for projects in which investigators do not have expertise, funding, or technical capabilities. . The modules are: Morphology, Tissue Culture/Monoclonal Antibody, Animal Resource, Laboratory Computer Applications, and Biostatistics. The Morphology Module will provide light and electron microscopy capabilities and tissue preparation for histochemistry and in situ hybridization, thereby increasing the availability of morphologic techniques to biochemists, pharmacologists, and immunologists who need correlative morphologic data for their research. The Tissue Culture/Monoclonal Antibody Module will assist PIs in selecting appropriate culture techniques and media and in producing characterized tissue cultures of ocular origin, and will be responsible for cell fusions and storage of designated clones for monoclonal antibody production. Such a resource will augment the research of investigators whose primary expertise and assistance in animal surgical techniques and postoperative care, administration of anesthesia, drugs, and medications, and antigen injections and bleedings required for polyclonal antibody production. The Laboratory Computer Applications Module will provide support to individual and collaborative efforts by assisting in interfacing of microcomputers with lab equipment and by developing software for gathering, processing, and analyzing experimental data by microcomputer systems. The Biostatistics Module will provide support to individual and collaborative efforts by assisting both clinicians and basic researchers in statistical aspects of study design and data analysis; principal module utilization will be in planning, design, and execution of clinical studies and in statistical analysis of research data. . The modules are staffed by personnel with specialized training in the respective fields. Each module is under the immediate supervision of an established, experienced investigator(s). The module heads constitute the Core Grant administrative committee, which is responsible to a committee of senior investigators who are ERI research unit heads.