Laser Microdissection Microscope

激光显微切割显微镜

• 批准号: 8826431
• 负责人: ILENE K. GIPSON
• 金额: $ 17.78万
• 依托单位: SCHEPENS EYE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8826431
• 关键词: Cells; Dissection; Ear; Eye; Fees; Foundations; Funding; Funding Applicant; Gases; Grant; Individual; Lasers; Longevity; Maintenance; Microdissection; Microscope; Microscopy; Microtomy; Minor; Morphology; Nitrogen; RNA; Research Institute; Research Personnel; Retina; Staging; System; Thick; Time; Training; United States National Institutes of Health; base; conjunctiva; cost; fluorescence imaging; instrument; solid state

 DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Leica Laser Microdissection 7000 system (LMD) to replace a Leica AS LMD system that is obsolete. The LMD 7000 is a state-of the art instrument that has a very high power, solid-state laser that allows the dissection capability needed to cut thick sections of the retina and conjunctiva. At the same time, the laser system has the ability to make fast precise cuts allowing efficient dissection and fine dissection of individual cells or groups of cells. The requested microscope would replace a Leica ASM that has a nitrogen gas laser that has a three year life span which loses cutting power over time, making dissection of thick sections impossible and multiple passes of the laser are needed to cut thinner sections. The gas laser has had to be replaced twice in the past 4 years, and weeks- to-months in downtime have been required for laser replacement and mirror recalibration. The quote for the requested Leica Laser Microdissection 7000 system with fluorescence imaging capabilities is $191,565. We will be able to use several objectives and a stage control system from the older system on the new system and this has decreased the request for funds by $6,184. Use of laser capture microscopy has grown dramatically at our research institute and currently 7 major users and 4 minor users request the new instrument. The investigators are funded by 7 NIH grants and a foundation grant. It is not feasible to use another instrument located at the Mass Eye and Ear Infirmary located 4-5 blocks away because of high internal use, availability of microscope time, as well as RNA isolation requirements. The microscope will be integrated into the Morphology Unit at the Schepens and will be over seen by Don Pottle who is currently responsible for the existing older LMD. Mr. Pottle is responsible for training new investigators in use of the instrument and its day-to-day maintenance and management. Use is based on signup in a first come first serve basis. The costs for maintenance of the instrument will be from fee for instrument use, which will be based on actual cost.

 描述（由适用提供）：要求购买Leica Laser Microssection 7000系统（LMD）的资金，以替换Leica为已过时的LMD系统。 LMD 7000是具有很高功率，固态激光器的最新仪器，可允许切除视网膜和结膜的厚部分所需的解剖能力。在 同时，激光系统具有快速削减的能力，可以有效解剖 以及单个细胞或细胞组的精细解剖。所需的显微镜将取代具有三年寿命的氮气激光器，该氮气激光器的生命周期会随着时间的流逝而失去切割动力，从而使对厚部分的解剖是不可能的，并且需要多次激光段来切割较薄的部分。在过去的4年中，必须将气体激光器替换两次，并且在停机时间内需要几周的时间才能进行激光置换和镜像重新校准。荧光成像功能的请求的Leica Laser Microdection 7000系统的报价为191,565美元。我们将能够使用新系统上旧系统的几个目标和舞台控制系统，这将资金的要求提高了6,184美元。我们的研究所的使用激光捕获显微镜已经急剧增长，目前有7位主要用户和4个未成年人请求新仪器。调查人员由7名NIH赠款和一项基金会赠款资助。由于内部用途高，显微镜时间的可用性以及RNA隔离要求，使用位于4-5个街区的大型眼睛和耳朵医院的另一种仪器不可行。显微镜将被整合到Schepens的形态学单元中，并将被目前负责现有旧LMD负责的Don Pottle看到。 Pottle先生负责培训新的调查人员使用该工具及其日常维护和管理。使用是基于先到先得的注册。维护仪器的成本将来自用于使用仪器的费用，这将基于实际成本。