Laser Microdissection Microscope

激光显微切割显微镜

• 批准号: 8826431
• 负责人: ILENE K. GIPSON
• 金额: $ 17.78万
• 依托单位: SCHEPENS EYE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8826431
• 关键词: Cells; Dissection; Ear; Eye; Fees; Foundations; Funding; Funding Applicant; Gases; Grant; Individual; Lasers; Longevity; Maintenance; Microdissection; Microscope; Microscopy; Microtomy; Minor; Morphology; Nitrogen; RNA; Research Institute; Research Personnel; Retina; Staging; System; Thick; Time; Training; United States National Institutes of Health; base; conjunctiva; cost; fluorescence imaging; instrument; solid state

 DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Leica Laser Microdissection 7000 system (LMD) to replace a Leica AS LMD system that is obsolete. The LMD 7000 is a state-of the art instrument that has a very high power, solid-state laser that allows the dissection capability needed to cut thick sections of the retina and conjunctiva. At the same time, the laser system has the ability to make fast precise cuts allowing efficient dissection and fine dissection of individual cells or groups of cells. The requested microscope would replace a Leica ASM that has a nitrogen gas laser that has a three year life span which loses cutting power over time, making dissection of thick sections impossible and multiple passes of the laser are needed to cut thinner sections. The gas laser has had to be replaced twice in the past 4 years, and weeks- to-months in downtime have been required for laser replacement and mirror recalibration. The quote for the requested Leica Laser Microdissection 7000 system with fluorescence imaging capabilities is $191,565. We will be able to use several objectives and a stage control system from the older system on the new system and this has decreased the request for funds by $6,184. Use of laser capture microscopy has grown dramatically at our research institute and currently 7 major users and 4 minor users request the new instrument. The investigators are funded by 7 NIH grants and a foundation grant. It is not feasible to use another instrument located at the Mass Eye and Ear Infirmary located 4-5 blocks away because of high internal use, availability of microscope time, as well as RNA isolation requirements. The microscope will be integrated into the Morphology Unit at the Schepens and will be over seen by Don Pottle who is currently responsible for the existing older LMD. Mr. Pottle is responsible for training new investigators in use of the instrument and its day-to-day maintenance and management. Use is based on signup in a first come first serve basis. The costs for maintenance of the instrument will be from fee for instrument use, which will be based on actual cost.

 描述（由申请人提供）：申请资金用于购买Leica Laser Microdissection 7000系统（LMD），以取代过时的Leica AS LMD系统。LMD 7000是一种最先进的仪器，具有非常高的功率，固态激光器，允许切割视网膜和结膜厚切片所需的切割能力。在 同时，激光系统具有快速精确切割的能力，从而实现有效的解剖 和精细解剖单个细胞或细胞群。所要求的显微镜将取代Leica ASM，后者的氮气激光器寿命为三年，随着时间的推移会失去切割能力，无法切割厚切片，需要多次通过激光才能切割更薄的切片。在过去的4年中，气体激光器不得不更换两次，并且需要数周至数月的停机时间来更换激光器和重新校准反射镜。所要求的徕卡激光显微切割7000系统与荧光成像功能的报价是191565美元。我们将能够在新系统上使用旧系统的几个物镜和一个舞台控制系统，这使资金需求减少了6，184美元。 激光捕获显微镜的使用在我们的研究所急剧增长，目前有7个主要用户和4个次要用户要求使用新仪器。研究人员由7个NIH赠款和一个基金会赠款资助。由于大量内部使用、显微镜时间的可用性以及RNA分离要求，使用位于4-5个街区外的大众眼耳医院的另一台仪器是不可行的。 该显微镜将被集成到Schepens的形态学单元中，并将由目前负责现有旧LMD的Don Pottle进行检查。Pottle先生负责培训新的研究人员使用仪器及其日常维护和管理。使用是基于先到先得的基础上注册。仪器的维护费用将来自仪器使用费，该费用将基于实际成本。