IMMUNOLOGICAL ASPECTS OF AGING

寿命受多种基因、性别和环境因素的影响。 环境因素的影响可以通过使用标准 包括当年出生日期在内的条件。 遗传因素 可以使用在特定染色体上不同的同类小鼠进行研究 区域，例如，在第四或第十七条染色体上。 许多表型变化 随着年龄的增长而发生;我们感兴趣的是那些影响免疫系统的因素。 系统 我们建议测试正在进行的研究表明，H-2d单倍型 与对淋巴瘤的抵抗力和维持T细胞免疫相关 活力，以及H-2和4号染色体近B基因座内基因影响 自然杀戮 我们还希望探测影响H2基因的 寿命位于H-2单倍型的D端。 此外，H-2 H-2末端控制对淋巴瘤的抗性， 功能协调发展的 我们将使用几个实验模型：a）B10 D2 N，B10 BR，B10 以及它们之间的杂交，以研究H-2与发病率的关系 淋巴瘤、T细胞功能和自然杀伤。 B）B10 D2N，B10，（ B10 D2 NxB 10 BR）F1和（B10 D2 NxB 10）F1，以研究逆转录病毒表达 使用RNA杂交北方印迹的序列能够预测 在不同H-2单倍型的小鼠中淋巴瘤的发生，以及c）我们将 比较正常脾淋巴细胞和淋巴细胞中I类基因产物 有淋巴瘤的结节。 d）比较寿命、肿瘤发病率和NK Bailey的Hw 26 c和C57 BL/6中的功能。 这些同源基因的区别仅在于 4号染色体上一个小区域标记为B（Hw 26 c具有C57 BL/6背景 和Balb/c的4号染色体的一部分）。 （二）建议的第二部分 打算绘制H-2对寿命、T细胞功能和 使用几种同源小鼠的H-2 D端淋巴瘤发生率 H-2等位基因的组合 例如，B10.A（H-2a）和B10.A（5 R） H-215仅在H-2的k端不同，而B10. B10.A（2R）.

Life span is influenced by several genes, sex and by environmental factors. The impact of environmental factors can be minimized by using standard conditions including the dated birth during the year. The genetic factors can be studied using congenic mice differing at specific chromosomal regions, e.g., on the 4th or 17th chromosomes. Many phenotypic changes occur with age; those we are interested in are those affecting the immune system. We propose to test ongoing studies suggesting that H-2d haplotype is associated with resistance to lymphomas and maintenance of T cell immune vigor, and that genes within H-2 and near b locus of chromosome 4 influence natural killing. We also wish to probe that the H-2 genes that influence life span are located in the D end of the H-2 haplotype. Also that the H-2 end of the H-2 controls resistance to lymphomas and both NK and T cell functions. We will use several experimental models: a) B10D2N, B10BR, B10 and hybrids among them to study the relationship between H-2 and incidence of lymphomas, T cell functions and natural killing. b) B10D2N, B10, ( B10D2NxB10BR)F1 and (B10D2NxB10)F1 to study the expression of retroviruses sequences using RNA hybridization Northern blots to be able to predict the occurrence of lymphomas in mice of different H-2 haplotypes and c) We will compare Class I gene products in normal lymphocytes of spleen and lymph nodes with lymphomas. d) Compare life span, incidence of tumors and NK functions in Hw26c and C57BL/6 of Bailey. These congenics differ only in a small region of chromosome 4 marked by b (The Hw26c have C57BL/6 background and part of chromosome 4 of Balb/c). e) The second part of our proposal intends to map the H-2 influence in life span, T cell function and incidence of lymphomas in the D end of H-2 using several congenics mice with combinations of H-2 alleles. For example, B10.A(H-2a) and B10.A(5R) H-2i5 differ only in the k end of H-2, and B10.A differ at D end of B10.A(2R).