Elucidating the on/off switch for an essential mitotic motor

这项研究的目的是发现细胞增殖是如何控制的，以及它可能如何开启和关闭。正如我们的身体有一个骨架为我们提供支持和力量一样，我们身体的细胞也有一个骨架--称为细胞骨架--它也提供支撑和力量。细胞骨架参与了细胞生命的许多重要方面，包括细胞的运动、结构和增殖。研究细胞骨架很重要，这样我们就可以了解健康细胞是如何工作的，也可以了解细胞骨架在疾病中的故障。我的研究团队研究细胞骨架的三维结构，因为了解细胞骨架是什么样子有助于我们理解它在细胞中是如何工作的。我们使用非常强大的显微镜来拍摄单个细胞骨架分子的照片，然后使用计算机将这些照片组合在一起，计算出它们的三维形状。我们目前的研究重点是细胞骨架的一部分，称为微管。这些长长的圆柱形结构就像细胞周围的轨道，细胞的转运蛋白马达沿着这些结构运动；这些马达被称为运动蛋白，有很多不同的类型。在这个项目中，我们将研究一种激动素-5，它对准确的细胞增殖非常重要。我们想知道Kinesin-5是如何利用细胞燃料沿微管移动的，以及这种活动是如何被控制的。我们最近从显微镜研究中得到了一些令人兴奋的结果，我们想更多地了解这种微小的、必不可少的马达是如何工作的。之前的实验，包括我们自己的研究，都表明Kinesin-5马达有一个开关，告诉它们什么时候移动，但我们还不太清楚它是如何工作的。我们新项目的目标是了解这种转换以及是什么控制了它。这一点尤其重要，因为我们知道癌症是由失控的细胞增殖造成的，所以我们也希望通过了解kinesin-5是如何开启和关闭自己的，我们或许能够为癌症的治疗提供更多的洞察力。

The purpose of this research is to discover how cell multiplication is controlled and how it might be turned on and off. In the same way as our bodies have a skeleton that provides us with support and strength, the cells of our bodies have a skeleton - called the cytoskeleton - which also provides support and strength. The cytoskeleton is involved in many important aspects of the life of the cell, including cell movement, architecture and multiplication. Studying the cytoskeleton is important both so we can understand how healthy cells work, but also to understand malfunctions of the cytoskeleton in disease. My research team studies the three-dimensional structure of the cytoskeleton, because knowing what the cytoskeleton looks like contributes to our understanding of how it works in the cell. We use a very powerful microscope to take pictures of individual cytoskeleton molecules and then use computers to combine these pictures and calculate their three-dimensional shape. Our current research focuses on a part of the cytoskeleton called microtubules. These are long cylindrical structures that act like tracks around the cell and along which the cell's transporter motors travel; these motors are called kinesins and there are lots of different types. In this project, we will be studying a kinesin type-5, which is very important for accurate cell multiplication. We want to know how kinesin-5s use cellular fuel to move along microtubules and how this activity is controlled. We have had some exciting recent results from our microscopy studies and we want to know more about how this tiny, essential motor works. Previous experiments, including our own research, have suggested that kinesin-5 motors have an on-off switch that tells them when to move, but we don't understand very well how this works. The aim of our new project is to understand this switch and what controls it. This is particularly important because we know that cancer results from out-of-control cell multiplication, so we also hope that by understanding how kinesin-5 switches itself on and off, we might be able to provide more insight into how cancer can be treated.