ANTIBODY FORMATION BY LYMPHOID CELLS

涉及T淋巴细胞的免疫应答在免疫系统中具有重要意义。 决定器官移植的命运，许多病毒感染的结果， 也可能是对肿瘤的反应。 T淋巴细胞与 在这些情况中的每一种情况下通过抗原特异性细胞表面 受体。 虽然大量的证据表明，T 细胞受体的独特型决定因素与那些发现于 免疫球蛋白和T细胞之间存在遗传联系 受体和免疫球蛋白重链同种异型，这个重要的细胞 受体仍然难以捉摸。 随着克隆T细胞的发展， 抗体生产杂交瘤技术，现在可以获得 同源T细胞和单克隆抗体。 我们将使用这些材料 目的研究溶细胞性T淋巴细胞（CTL）的抗原受体。 我们 已经获得了“克隆特异性”单克隆抗体，我们将开发 另外的单克隆“克隆特异性”抗体， 克隆的CTL的抗原相关功能。 这种抗体很可能 与抗原的T细胞受体反应的抗体的候选物。 我们还将开发与CTL同种抗原反应的单克隆抗体 与免疫球蛋白（IG）重链连接的IgT-CCTL 同种异型 这些抗体可能是抗体反应性的候选者。 T细胞受体上的“恒定区”标记。 的 “克隆特异性”和“IgT-CCTL”抗体以及与其结合的CTL react将用于与利根川进博士的合作研究， 鉴定和表征编码以下抗原受体的基因： 溶细胞性T细胞。 我们还将试图确定CTL和 与相同同种异体抗原表位反应的抗体具有相同的 独特型 拟议研究的最终目标是获得 更好地了解T细胞的功能特征 受体，开发更特异、合理、有效的 操纵特定免疫反应的手段 虽然老鼠被 在这些研究中，我们认为所获得的信息可能是 最终应用于人类。

Immune responses involving T lymphocytes are of major importance in determining the fate of organ grafts, the outcome of many virus infections, and probably the response to tumors as well. T lymphocytes react with antigens in each of these situations through antigen-specific cell surface receptors. Although a considerable body of evidence indicates that the T cell receptor shares idiotypic determinants with those found on immunoglobolin and that there is genetic linkage between the T cell receptor and the immunoglobulin heavy chain allotype, this important cell receptor has remained elusive. With the development of cloned T cells and antibody-producing hybridoma technology, it is now possible to obtain homogeneous T cells and monoclonal antibodies. We will use these materials to characterize the antigen receptor of cytolytic T lymphocytes (CTL). We have obtained a "clone-specific" monoclonal antibody, and we will develop additional monoclonal "clone-specific" antibodies which inhibit the antigen-related functions of cloned CTL. Such antibodies are likely candidates for antibodies reactive with the T cell receptor for antigen. We also will develop monoclonal antibodies reactive with CTL alloantigens ("IgT-CCTL") which are linked with immunoglobulin (Ig) heavy chain allotypes. Such antibodies are likely candidates for antibodies reactive with "constant region" markers on the T cell receptor. The "clone-specific" and "IgT-CCTL" antibodies and the CTL with which they react will be used in collaborative studies with Dr. Susumu Tonegawa to identify and characterize genes coding for the antigen receptors of cytolytic T cells. We also will try to determine whether CTL and antibodies reactive with the same alloantigenic epitope bear the same idiotype. The ultimate objectives of the proposed studies are to gain a better understanding of the functional characteristics of the T cell receptor for antigen and to develop more specific, rational, and effective means to manipulate specific immune responses. Although mice are being used in these studies, we feel that the information obtained may be applicable ultimately in human situations.