GENETICS OF LAMBDA PROPHAGE--BIOTIN OPERON

长期目标是了解病毒如何与病毒相互作用 染色体和蛋白质生物合成所需的基因 少量受到监管。这些与健康相关的东西 原核生物模型体系的研究在于阐明 自然生物学的基本机制和观点 从细菌的共同特征看致病病毒 和动物病毒学。噬菌体Lambda的DNA上游 整合酶操纵子的影响将被检测 两个元素的整合和删减：终结者式的 与PI启动子和开放阅读框架相邻的序列ti‘ (ORF-55)它的上游。为消除ti‘而构建的噬菌体 将检查CII蛋白在存在的情况下的效果 转录自Pl，以检验ti‘在 从未承诺状态到溶原性状态的初始转变。 从一个质粒表达的ORF-55基因将被测试 Lambda CRG切除酶缺陷的补充 噬菌体，在ORF-55中有一个IS2插入。拉姆达CRG 倒置方向的预言体将被测试是否有能力 通过与有缺陷的GSR‘重组来生成复制 噬菌体，以及大肠杆菌菌株的缺陷前驱体 将对EcoR集合进行比较。纯化后的E. 一种新的大肠杆菌生物素亚砜还原酶及其分子基础 进化的BDS还原酶活性将被确定。

The long term goals are to understand how viruses interact with chromosomes and how genes for biosynthesis of proteins needed in small amounts are regulated. The health relatedness of these studies on prokaryotic model systems lies in the elucidation of basic mechanisms and in the perspective shed on natural biology of disease-causing viruses by the common features of bacterial and animal virology. The DNA of bacteriophage lambda upstream of the integrase operon will be examined for effects on integration and excision of two elements: a terminator-like sequence tI' that abuts the pI promoter and an open reading frame (ORF-55) upstream from it. Phage constructed to eliminate tI' will be examined for the effect of cII protein in the presence of transcription from pL, to test the hypothesis that tI' functions in the initial transition from the uncommitted to the lysogenic state. The ORF-55 gene expressed from a plasmid will be tested for complementation of the excisionase defect of the lambda crg phage, which has an IS2 insertion in ORF-55. Lambda crg prophages in inverted orientation will be tested for the ability to generate duplications by recombination with the defective gsr' prophage, and the defective prophages of E. coli strains of the ECOR collection will be compared. The properties of purified E. coli biotin sulfoxide reductase and the molecular basis for a newly evolved BDS reductase activity will be determined.