ORIGIN OF LYMPHOCYTES IN THE EMBRYONIC OMENTUM

拟议的研究旨在确定发展中的 小鼠胚胎早期大网膜雏形的形成潜力 淋巴细胞。初步研究表明，腹股沟的大网膜原基 早期的小鼠胚胎含有可以形成淋巴细胞的干细胞，而且它是 拟议研究工作的目的是确定 并确定它们的发育来源和 它们在免疫功能中的后续作用。 具体的实验目标包括：1)分析形成的淋巴样细胞 通过移植或体外培养13天小鼠的大网膜雏形 (分化标记、凝集素结合特性、器官特异性 抗原；体内和体外的功能研究；集落形成 2)大网膜的组织发生和形态发生的研究 从小鼠发育到出生的第10天，包括体内和 干细胞群体的体外特征；以及3)研究 遗传性眼底发育不良的研究进展 造血(W系列)、内脏中胚层(dh/+、dh/dh)、胸腺 (Nu/Nu)或B细胞成熟(Cba/Nxid)系统。 拟议研究的基本目标是研究一种新的来源 胚胎淋巴细胞，以努力更好地了解 淋巴系统个体发育的一般情况，并希望分析 可能定义了一种特殊的，可能是新的 在胎儿和/或成人免疫功能中起重要作用的细胞亚群。

The proposed research is directed at determining the developmental potential of the early omental rudiment of the mouse embryo to form lymphocytes. Preliminary studies indicate that the omental anlage of the early mouse embryo contains stem cells that can form lymphocytes, and it is the purpose of the proposed research work to characterize the omentum-derived cells and to determine both their developmental origin and their subsequent role in immune functions. Specific experimental aims include: 1) Analysis of lymphoid cells formed by the 13-day mouse omental rudiment in transplants or in vitro (differentiation markers, lectin-binding properties, organ-specific antigens; functional studies in vivo and in vitro; colony forming potential); 2) study of histogenesis and morphogenesis of the omental anlage from day 10 of mouse development to birth, including the in vivo and in vitro characterization of stem cell populations; and 3) investigation of the development of omental rudiments with genetic impairment of the hematopoietic (W series), splanchnic mesodermal (Dh/+,Dh/Dh), thymic (nu/nu) or B-cell maturation (CBA/N xid) systems. The basic goal of the proposed research is to study a new source of embryonic lymphocytes in an effort to gain greater understanding of the ontogeny of lymphoid systems in general, and in the hope that the analysis of omentum-derived lymphocytes may define a specific and possibly new subset of cells important in fetal and/or adult immune functions.