IMMUNITY IN TRANSGENIC MICE

β细胞被其同源抗原刺激会引发一系列的免疫反应。 抗体开发中的增殖和分化步骤- 分泌浆细胞 在分子水平上，两个关键过程可以 改变编码β细胞抗体的功能性抗体基因 重链 重链基因可以经历类别转换， 体细胞超突变 我们发现微量注射的伽马射线 链基因也可以发生同种型转换和体细胞突变， 转基因小鼠 我们将分析转基因小鼠，以确定 转基因同种型转换的机制， 在允许转换过程中重要的核苷酸序列。 我们还将分析顺式作用序列，这些序列在 将超突变机制导向重链转基因，和 RNA转录是否在超突变过程中发挥作用。

The stimulation of a beta-cell by its cognate antigen triggers a series of proliferation and differentiation steps in the development of an antibody- secreting plasma cell. At the molecular level, two key processes can alter the functional antibody gene that encodes the beta-cell antibody heavy chain. Heavy chain genes can both undergo class switching and somatic hypermutation. We have found that a microinjected gamma heavy chain gene can also undergo isotype switching and somatic mutation in transgenic mice. We will analyze transgenic mice to determine the mechanisms that are involved in transgene isotype switching and the nucleotide sequences that are important in allowing the switching process. We will also analyze the cis-acting sequences that are important in directing the hypermutational mechanism to a heavy chain transgene and whether RNA transcription plays a role in the hypermutation process.