CHARACTERIZATION/TICK ANTIGENS ELICITING HOST IMMUNITY

在这份赠款提案中，提出了确定哪个扁虱的研究报告 (Amblyomma Americanum)唾液腺抗原是引起 诱导和激发宿主免疫抗性。各种唾液 腺体来源的物质将从活的扁虱中收集并进行检测 体外和体内抗原性研究。这些研究的结果 研究将导致分离和表征 不同层析方法检测耐药相关抗原 电泳法。这样的研究将阐明 抗原的产生和释放。一旦关键的壁虱抗原进入体内 另一方面，这些物质将被测试它们免疫宿主的能力 对抗扁虱。由于我们已经有了纯的抗蜱抗体(7SIgG1)在 另一方面，使用这种重要的试剂作为探针将有助于我们 寻找关键的壁虱抗原。此外，我们计划利用鼠标作为 扁虱宿主产生针对不同扁虱的单抗 抗原。这些高度特异的抗体的产生将会增加 我们的报告能够从粗制的壁虱中分离出关键的抗原 萃取物。关键抗原(S)在手，形成最终的氨基酸 测序，并最终将相应的基因插入到 细菌，有可能实现大规模的抗原生产 用于牛的疫苗的开发。利用这些较新的 技术是需要多年复杂工作的大步骤，但 关键的第一步已经完成，那就是分离特定的 传递免疫抵抗力的抗体(7SIgG1)，可能是单特异性的 一种关键的壁虱抗原。建议的研究涵盖以下学科： 昆虫学、寄生虫学、免疫学和分子生物学。尽管 拟议的研究是复杂和复杂的，这些不同的领域 研究在我们的能力范围内，并有新的理论和信息 应该产生实际的重要性。

In this grant proposal, studies are presented to determine which tick (Amblyomma americanum) salivary gland antigens are responsible for the induction and elicitation of host immune resistance. Various salivary gland derived substances will be collected from live ticks and assayed for antigenicity by in vitro and in vivo techniques. The results from these studies will lead to the isolation and characterization of the resistance-associated antigens by various chromatographic and electrophoretic techniques. Such studies will elucidate the kinetics of antigen production and release. Once the crucial tick antigens are in hand, these substances will be tested for their ability to immunize hosts against ticks. Since we already have pure anti-tick antibody (7SIgG1) in hand, the use of this important reagent as a probe will facilitate our search for crucial tick antigens. In addition, we plan to utilize mice as tick hosts to generate monoclonal antibodies directed at various tick antigens. The production of these highly specific antibodies will increase our reportoire of probes able to isolate crucial antigens from crude tick extracts. With the crucial antigen(s) in hand for eventual amino acid sequencing, and eventual insertion of the corresponding genes into bacteria, it may be possible to achieve large scale antigen production for vaccine development for use in cattle. Exploitation of these newer technologies are big steps that require years of complex work, but the essential first step has been achieved, that is the isolation of specific antibody (7SIgG1) that transfers immune resistance and may be monospecific for a crucial tick antigen. The studies proposed span the disciplines of entomology, parasitology, immunology and molecular biology. Although the proposed studies are complex and sophisticated, these diverse areas of research are within our ability and new information of theoretical and practical importance should be generated.