MONOCLONAL HUMAN LUPUS AUTOANTIBODIES

杂交瘤技术将被用于研究细微的特异性和 人类狼疮自身抗体的独特型相关性。我们会做好准备 诱变的人骨髓瘤细胞与B淋巴细胞融合的杂交瘤 从系统性红斑狼疮患者的血液中提取。杂交瘤 将对产品进行筛选，以确定其结合DNA、组蛋白和 心磷脂。我们将描述杂交瘤的细微特异性。 通过竞争性免疫分析检测自身抗体。我们还将测试 单克隆性狼疮自身抗体与神经细胞结合的能力 淋巴细胞。将通过免疫来制备抗独特型抗体 具有杂交瘤自身抗体的兔子。然后我们将分析独特型 一组杂交瘤自身抗体之间的相关性，并通过 免疫测定法检测狼疮患者血清独特型水平及其临床意义 直系亲属。淋巴细胞表面的独特型将通过 荧光素标记的抗独特型血清。最后，细微的细节和 药物相关患者自身抗体的独特型相关性 将对狼疮进行研究，并将其与来自 自发性狼疮。

Hybridoma technology will be employed to study the fine specificities and idiotypic relatedness of human lupus autoantibodies. We will prepare hybridomas by fusion of mutagenized human myeloma cells with B lymphocytes from the blood of patients with systemic lupus erythematosus. Hybridoma products will be screened for their ability to bind DNA, histones, and cardiolipin. We will characterize the fine specificities of the hybridoma autoantibodies by means of competitive immunoassays. We will also test the ability of monoclonal lupus autoantibodies to bind to neuronal cells and to lymphocytes. Anti-idiotypic antibodies will be prepared by immunization of rabbits with the hybridoma autoantibodies. We will then analyze idiotypic relatedness among a panel of hybridoma autoantibodies and measure, by immunoassay, serum levels of the idiotypes in lupus patients and their immediate relatives. Idiotypes on lymphocyte surfaces will be sought with fluorescein-tagged anti-idiotypic sera. Finally, fine specificities and idiotypic relatedness of autoantibodies from patients with drug-induced lupus will be studied and compared with those from patients with spontaneous lupus.