HUMAN IMMUNE COMPLEX DISEASES

关于免疫复合体在体内的作用的先前工作 系统性红斑狼疮(SLE)的发病机制 关节炎和相关疾病将继续存在。在对…的研究中 RA、混合性结缔组织病与本质混合性 冷球蛋白血症(EMC)--类风湿因子在血液病中的作用 和佤邦杂交独特型(XIds)将被调查。多克隆 携带BLA xid的RFS似乎在#年高发 类风湿性关节炎，而患有风湿性关节炎的人广泛发生 在EMC和原发性干燥综合征中。RF BLA组是 除与免疫球蛋白反应外，还能与DNA核蛋白发生反应。在……里面 RA将重点放在免疫的特征上 含有BLA RFS的复合体。使用敏感的免疫印迹 非免疫球蛋白抗原与RF和AND交叉反应技术 将寻求抗独特型抗体。类似的研究将会是 对EMC患者的冷冻球蛋白和 将检验抗独特型抗体参与的假说 早期补体成分激活的模式 这种病。新研制的小鼠抗WAXID和WAXID单抗 在这些研究中，将在模型系统上使用反WAID。在……里面 SLE的主要重点将是研究血清低水平 确定与其关系的相对分子质量Clq反应物 与低补体血症相关的天然抗体 这些反应物患者体内出现的DNA。新的尝试 将研制出一种抗天然DNAXID抗体 只与nDNA抗体反应。这样的试剂将会 极大地促进了这些研究。

Previous work on the role of immune complexes in the pathogenesis of systemic lupus erythematosus (SLE), rhematoid arthritis and related disorders will be continued. In studies on RA, mixed connective tissue disease and essential mixed cryoglobulinemia (EMC), the role of rheumatoid factors with BLA and WA cross idiotypes (XIds) will be investigated. Polyclonal RFs bearing the BLA XId appear to occur in high incidence in rheumatoid arthritis while those bearing the WA XId occur widely in EMC and primary Sjogren syndrome. The BLA group of RFs are unique in reacting with DNA nucleoprotein in addition to IgG. In RA emphasis will be placed on characterization of immune complexes containing BLA RFs. Using sensitive immunoblotting techniques non-IgG antigen cross reactive with RF and antiidiotype antibodies will be sought. Similar studies will be done on the cryoglobulin from patients with EMC and the hypothesis will be tested that antiidiotype antibody is involved in the pattern of early complement component activation seen in this disease. Newly developed mouse monoclonal anti WA XId and anti WA Id will be used on a model system in these studies. In SLE the primary emphasis will be on studying the serum low molecular weight Clq reactants to determine their relationship to the associated hypocomplementemia and antibodies to native DNA that occurs in patients with these reactants. New attempts will be made to develop an anti native DNA XId antibody that reacts only with nDNA antibodies. Such a reagent would markedly facilitate these studies.