PHYSIOLOGY OF HYPOTHALAMIC NEUROHORMONES

我们建议继续我们目前的下丘脑生理学项目， 调节垂体激素分泌的神经激素。 重点将 放置在描绘释放生长激素释放激素（GHRH）， 催乳素释放激素（PRH）和皮质醇释放激素（CRH） 大鼠垂体门静脉血，并确定这些特征 下丘脑激素 将测量垂体激素释放活性 使用多通道灌注系统，然后测量垂体 通过适当的RIA测定灌注输出中的激素。 如果这些活动 存在于大鼠垂体门静脉血中，我们将评估激素 门静脉血的释放活动后，电刺激各种 已知刺激相应垂体释放的大脑区域 激素和脑室内注射各种神经递质。 这将 作为对生理重要性的初步评估 观察到的激素释放活性，并将提供释放富含激素的 门静脉血 然后将富集的门静脉血汇集、提取， 检查的活性物质的理化特性。 并行 研究中，从以下来源获得的活性物质的理化性质 大鼠和家畜的下丘脑提取物以及大鼠垂体的提取物 将比较门静脉血。 如果发现任何馏分含有显著 GHRH、PRH或CRH活性，将努力分离这些激素。 来自大鼠和家畜的胰腺和肠组织的提取物也将 检查这些释放活动。 如果任何下丘脑外部分 如果发现具有这些活性，将努力分离这些活性， 物质，及其物理化学性质相比，那些积极的 在大鼠垂体门静脉血和下丘脑提取物中发现的物质。

We propose to continue our current project on the physiology of the hypothalamic neurohormones which regulate secretion of pituitary hormones. Emphasis will be placed on delineating the release of growth hormone releasing hormone (GHRH), prolactin releasing hormone (PRH), and corticotronin releasing hormone (CRH) into the rat hypohyseal portal blood and determining characteristics of these hypothalamic hormones. Pituitary hormone releasing activity will be measured using a multichannel perifusion system followed by measurement of the pituitary hormones in the perifusion output by the appropriate RIA. If such activities are present in the rat hypophyseal portal blood, we will assess the hormone releasing activity of portal blood following electrical stimulation of various regions of the brain known to stimulate the release of the respective pituitary hormone and intraventricular injection of various neurotransmitters. This will serve as a preliminary assessment of the physiological importance of the observed hormone releasing activity and will provide releasing hormone-enriched portal blood. The enriched portal blood will then be pooled, extracted and the physicochemical characteristics of the active substances examined. In parallel studies, the physico-chemical properties of active substances obtained from hypothalamic extracts of rats and domestic animals and from rat hypophyseal portal blood will be compared. If any fraction is found to contain significant GHRH, PRH, or CRH activity, an effort will be made to isolate these hormones. Extracts of pancreatic and gut tissue from rats and domestic animals will also be examined for these releasing activities. If any extrahypothalamic fraction is found to possess these activities an effort will be made to isolate these substances, and their physico-chemical properties compared to those of active substances found in rat hypophyseal portal blood and hypothalamic extracts.