BIOCHEMICAL ASPECTS OF RENAL ELECTROLYTE TRANSPORT

这项建议是为了研究药物的肾脏处置。行动 肾脏上的药物，以及药物的相互作用。这些子项目包括：1)研究 对药物的主动重吸收机制的研究(S)。所提出的问题如下： 不同药物之间是否存在相互抑制转运的现象？ 哪些是积极重新吸收的？这种再吸收机制是否与 尿酸的作用机制？活性重吸收通量能被证明为 尿酸类药物在清除实验中似乎不会被重新吸收？ 2)唑唑胺的尿酸作用。问题是：有机食品能否 碱本身是尿酸的，还是必须在体内转化为阴离子 代谢物。3)氯噻嗪的降钙作用。问题是： 由于酶的抑制而产生的低热量作用(S) 磷酸二酯酶？4)碳酸酐酶的大分子抑制剂。这个 问：腔内碳酸酐酶在体内的定量作用是什么？ 重吸收重碳酸盐？ 这些实验将在狗、黑猩猩、Cebus猴子和大鼠身上进行。 主要技术有肾清除、阻断血流、显微穿刺术、 微灌注术。

This proposal is for the study of the renal disposition of drugs. The actions of drugs on the kidney, and drug interactions. The subprojects are: 1) A study of the active reabsorptive mechanism(s) for drugs. The questions addressed are: Can mutual inhibition of transport be demonstrated among the various drugs which are actively reabsorbed? Is this reabsorptive mechanism the same as the mechanism for uric acid? Can active reabsorptive fluxes be demonstrated for uricosuric drugs which do not seem to be reabsorbed in clearance experiments? 2) The uricosuric action of zoxazolamine. The question is: Can an organic base per se be uricosuric or must it be converted in vivo to an anionic metabolite. 3) The hypocalicuric action of chlorothiazide. The question: Is the hypocalcuric action a result of inhibition of the enzyme(s) phosphodiesterase? 4) A macromolecular inhibitor of carbonic anhydrase. The question: What is the quantitative role of lumenal carbonic anhydrase in the reabsorption of bicarbonate? The experiments will be conducted in dogs, chimpanzees, Cebus monkeys and rats. The major techniques are renal clearance, stop-flow, micropuncture, microperfusion.