CELL BIOLOGY OF GLOMERULAR ULTRAFILTRATION

调节肾小球滤过率的能力对于 肾脏功能正常。近几年来，很明显， 肾小球壁和/或通透性的显著改变 可用于超滤的表面积(即超滤 系数)可与多种重要的荷尔蒙反应， 肾毒性化合物，以及其他改变的生理状态。目前， 与肾小球细胞和形态变化有关的 这些超滤系数的变化是非常推测的。 或者是未知的。在拟议的研究中，体外和体内研究 旨在仔细评估细胞的形态反应 肾小球上皮足细胞、肾小球内皮细胞和 肾小球间皮细胞在改变超滤的情况下 慕尼黑-Wistar大鼠的系数。我们将特别注意 细胞质收缩因子、细胞骨架因子、 钙离子和前列腺素在影响超微结构变化中的作用 在这些牢房里。综上所述，相信上述研究将会 提供对不同组织所扮演的角色的重要见解 肾小球在调节滤液流动中的构成类型 穿过肾小球壁，构成其基础的细胞生物学参数 超滤系数与这些细胞的形态变化有关， 并将有助于确定许多重要化合物的作用机制 和条件。

The ability to regulate glomerular filtration rate is essential for the normal functioning of kidneys. In recent years, it has become evident that significant alterations in the permeability of the glomerular wall and/or surface area available for ultrafiltration (i.e. the ultrafiltration coefficient) can occur in response to a wide variety of important hormones, nephrotoxic compounds, and other altered physiological states. At present, the glomerular cells and morphological changes which are responsible for these alterations in the ultrafiltration coefficient are very speculative or unknown. In the proposed investigations, in vitro and in vivo studies are designed to carefully evaluate the morphological responses of glomerular epithelial podocytes, the glomerular endothelium, and the glomerular mesothelial cells in situations which alter the ultrafiltration coefficient in Munich-Wistar rats. Particular attention will be paid to the roles that cytoplasmic contractile elements, cytoskeletal elements, calcium ions, and prostaglandins play in affecting ultrastructural changes in these cells. Together, it is believed that the above studies will provide important insights into the roles played by the different cell types which make up the kidney glomerulus in regulating flow of filtrate across the glomerular wall, the cell biological parameters which underlie ultrafiltration coefficient related morphological changes in these cells, and will help define the mechanisms of action of many important compounds and conditions.