THICK FILAMENT MYOSIN AND C-PROTEIN ARRANGEMENT

肌球蛋白和辅助蛋白如C蛋白在肌细胞中的排列 脊椎动物的横纹肌粗丝是不完全知道。 最近 然而，对青蛙粗细丝结构的实验， 证明脊椎动物粗丝的结构可能是 适合于通过电子显微镜、光学显微镜和光学显微镜的组合进行分析。 衍射分析和计算机图像重建技术。 的 拟议研究的具体目标是扩大这些研究的 青蛙粗丝，并扩大研究，以确定和 从鱼，禽， 和哺乳动物横纹肌。 这些研究的主要目标是 确定肌球蛋白的排列和数量， 这些细丝中的交叉桥水平以及C蛋白如何相对排列 与肌球蛋白的联系 实现这些目标的研究将 包括：1）制定程序，隔离土著人， 粗丝，每根纤维的结构变化最小， 2）电子显微镜和光学显微镜相结合的使用 对显微照片进行衍射分析，以评估细丝 保存;和3）使用计算机图像分析的长丝 在最好的电子显微镜图像，以确定旋转 肌球蛋白头的对称性和排列，以及 C蛋白 这些研究将提供关于以下方面的基本科学信息： 肌肉的结构，从而将提供更好的基础， 了解病理条件如何影响这种功能， 组织.

The arrangement of myosin and accessory proteins such as C-Protein in the vertebrate striated muscle thick filament is incompletely known. Recent experiments on the structure of frog thick filaments, however, have demonstrated that the structure of vertebrate thick filaments may be amenable to analysis by a combination of electron microscopy, optical diffraction analysis, and computer image reconstruction techniques. The specific aim of the proposed research is to expand these studies of the frog thick filaments and to extend the studies to the determination and comparison of the structure of thick filaments isolated from fish, avian, and mammalian striated muscle. The major objective of these studies will be the determination of the arrangement and number of myosins per crossbridge level in these filaments and how C-protein is arranged relative to the underlying myosin. The studies to accomplish these aims will include: 1) the development of procedures fro the isolation of native thick filaments with minimal structural alteration from each of the species; 2) the use of combination of electron microscopy and optical diffraction analysis of the micrographs to assess the filament preservation; and 3) the use of computer image analysis of the filament images in the best electron micrographs to determine the rotational symmetry and arrangement of the myosin heads, and the arrangement of C-protein. These studies will provide basic scientific information about the structure of muscle, and will thus provide a better foundation for understanding how pathologic condition may affect the functioning of this tissue.