WATER AND ELECTROLYTE MOVEMENT IN RENAL TUBULES

哺乳动物中各种结构-功能关系的描绘 Nephron代表了这一提议的主要研究目标。大多数细分市场 该方案涉及一项形态学-生理学联合调查 处理和使用各种实验室技术，包括传播 电子显微镜，扫描电子显微镜，光学显微镜， 显微穿刺术、离体管段微灌注术、冷冻骨折 电子显微镜和放射自显影。具体项目包括 兔近端有机碱分泌物的精确定位 小管、质膜和结合部的冷冻断裂检查 近端小管复合体在正常情况下和在 旨在增强细胞旁反渗漏的生理动作 分流途径，近端吞噬机制的检查 上肢粗大小管冷冻骨折检查的比较 延髓旁升粗肢的超微结构 浅层肾单位，检查肾脏的形态反应 肾小球引起肾脏灌注压的主要变化 改变那段肾单位的超滤特性， 未患病哺乳动物肾脏的继续详细形态分析 使用扫描电子显微镜的主要模式，并对其进行评价 致密斑作为肾素释放控制机制中的受体，以及作为 肾小球滤过的反馈控制机制中的受体 单肾单位水平。

The delineation of various structural-functional relationships in the mammalian nephron represents the major research goal of this proposal. Most segments of the protocol involve a combined morphological-physiological investigative approach and employ a variety of laboratory techniques including transmission electron microscopy, scanning electron microscopy, light microscopy, micropuncture, microperfusion of isolated tubule segments, freeze-fracture electron microscopy, and radioautography. Specific projects include the delineation of the precise site of organic base secretion in the rabbit proximal tubule, freeze-fracture examination of the plasma membranes and junctional complexes of the proximal tubule under normal conditions and during physiological maneuvers designed to enhance backleak through the paracellular shunt pathway, examination of the mechanism of phagocytosis in the proximal tubule, freeze-fracture examination of the ascending thick limb, comparison of the ultrastructural configuration of the ascending thick limb of juxtamedullary and superficial nephrons, examination of the morphological response of the renal glomerulus to major alterations in renal perfusion pressure which are known to alter the ultrafiltration characteristics of that segment of the nephron, continued detailed morphological analysis of the nondiseased mammalian kidney using the primary mode of scanning electron microscopy, and evaluation of the macula densa as a receptor in the mechanism controlling renin release, and as a receptor in the mechanism of feedback control of glomerular filtration at the single nephron level.