Sensor Integrated Biosensors (SIBs)

我们建议开发传感器集成生物反应器(SIB)作为一种工具，使研究人员和组织结构的生产者能够在长期培养(长达28天)期间无损地监测它们的发育。这一方案的创新之处在于将先进的传感、成像和监测技术融入现有的最先进的生物反应器系统：准活体生物反应器系统。增加的功能将允许对组织结构进行非侵入性的现场监测，从而避免在每个时间点牺牲实验来收集数据的需要。SIB将由该联盟中建立的最终用户组开发，以加快再生医学产品的开发周期，并通过提供比目前可用的更严格控制的体外模型来改善生产经济性。该项目将提供细胞培养系统，提供更快的组织生长，更准确的表型控制，并通过在流过系统中非破坏性地监控组织发育的能力来提高效率。该系统将在使用RegenTec的“可注射骨”支架的特定骨再生应用中得到验证。然而，在该项目中开发的包含传感器集成生物反应器(SIB)技术的准活体生物反应器将有更广泛的应用范围，而不仅仅是骨再生。生物反应器的初始尺寸将是研究应用的理想尺寸，但已经证明，多达36个准活体生物反应器可以并行操作，使它们固有的可伸缩性，用于临床用途的干细胞繁殖。

We propose to develop sensor integrated bioreactors (SIBs) as a tool enabling researchers and producers of tissueconstucts to monitor their development non-destructively during long-term culture (up to 28 days). The innovation in thisproposal is the incorporation of advanced sensing, imaging and monitoring technologies into an existing state-of-the-artbioreactor system: Quasi-Vivo. The added functionality will allow for non-invasive, in situ monitoring of tissue constructswhich will avoid the need to sacrifice experiments to collect data at each time point. The SIBs will be exploited by the endusergroup established in this consortium, both to speed the development cycle of regenerative medicine products andimprove the economics of production by providing a more closely controlled in-vitro model than is currently available.This project will deliver cell culture systems that provide faster growth of tissue, more accurate control of phenotype andimproved efficiency through the ability to monitor tissue development non-destructively in a flow-through system. Thesystem will be validated in a particular application for bone regeneration using RegenTec's ' injectable bone' scaffold.However the Quasi-Vivo bioreactors incorporating the Sensor Integrated Bioreactors (SIBs) technology developed in thisproject will have a wider range of application than just bone regeneration. The initial size of the bioreactor will be ideal forresearch applications but it has already been demonstrated that up to 36 Quasi-Vivo bioreactors can be operated in parallelmaking them inherently scaleable for stem cell propagation for clinical use.