A functional dissection of the bacterial replisome

该项目旨在研究复制体，即在细胞分裂前复制DNA的机器。多年的生物化学研究已经揭示了它的蛋白质成分是如何工作的，但需要在单分子水平上进行观察，才能理解它们是如何协同工作的。该项目旨在将新型单分子生物物理工具与最先进的生物化学相结合，以确定细菌复制体如何协调两条DNA链的合成以及它如何在飞行中交换蛋白质成分。该项目的预期成果包括提高对基本生物过程的理解，发展新的生物物理方法，以及培养下一代跨学科科学家。

This project aims to study the replisome, the machine that duplicates DNA before cell division. Years of biochemical research has shown how its protein components work, but observation at the single-molecule level is needed to understand how they all work together. This project aims to combine novel single-molecule biophysical tools with state-of-the-art biochemistry to define how the bacterial replisome coordinates synthesis of the two DNA strands and how it exchanges protein components on the fly. Expected outcomes of this project include improved understanding of a fundamental biological process, development of novel biophysical methodology, and training of the next generation of interdisciplinary scientists.