MECHANISM OF HORMONE ACTION ON TARGET CELLS IN CULTURE

雌激素(E)敏感的人乳腺肿瘤细胞(MCF7， T47D)是#年研究的中心问题。 这个实验室。雌二醇-17β(E_2)对特异性蛋白的影响 这些E2靶标的合成及其与增殖的关系 细胞的特征正在被确定。这些细胞携带雌激素并增殖 裸鼠注射雌二醇组比注射雌二醇组更快 缺乏雌二醇。在培养条件下，木炭-葡聚糖剥离人 血清抑制这些细胞的增殖，呈剂量依赖性； 雌激素取消了这种抑制作用。这些数据和其他数据是兼容的 以间接和消极的方式控制病毒的扩散 对雌激素敏感的细胞。这笔赠款的具体目的是净化 雌激素敏感型人细胞增殖的特异性抑制物 (雌酮)，并测定三苯基乙烯的作用。 (“抗雌激素”)对上述细胞增殖的影响 间接和直接负面控制范式的背景。(D)

The proliferation of estrogen (E)-sensitive human breast tumor cells (MCF7, T47D) in an "in animal-in culture" system is the central issue studied in this lab. The effect of estradiol-17 beta (E2) on specific protein synthesis and its relation with the proliferation of these E2 target cells is being characterized. These cells carry estrophilins and multiply faster in nude mice to which E2 is administered than in those in which E2 is lacking. In culture conditions, charcoal-dextran stripped human sera inhibit the proliferation of these cells in dose-dependent pattern; estrogens cancel this inhibition. These and additional data are compatible with the notion of an indirect and negative control of the proliferation of E2-sensitive cells. The specific aims of this grant are to purify the specific inhibitors of the proliferation of E2-sensitive human cells (estrocolyones) and determine the effect of triphenylethylenes ("antiestrogens") on the proliferation of the above mentioned cells within the context of the indirect and direct negative control paradigm. (D)