HUMAN IMMUNE COMPLEX DISEASES

以前的工作中的免疫复合物的作用， 系统性红斑狼疮（SLE）的发病机制 关节炎和相关疾病将继续治疗。 研究的 RA，混合性结缔组织病和原发性混合性 冷球蛋白血症（EMC），类风湿因子与BLA的作用 和WA交叉独特型（XIds）将被研究。 多克隆 携带BLA XId的RF似乎发生率较高， 类风湿性关节炎，而那些轴承WA XId发生广泛 在EMC和原发性干燥综合征。 RF的BLA组是 除了IgG之外，还独特地与DNA核蛋白反应。 在 RA的重点将放在表征免疫 含有BLA RF的复合物。 使用灵敏的免疫印迹 技术非IgG抗原与RF交叉反应， 将寻找抗独特型抗体。 类似的研究将 对EMC患者的冷冻蛋白进行了研究， 将检验抗独特型抗体参与 早期补体成分激活的模式见于 这种疾病。 新开发的小鼠单克隆抗WA XId和 在这些研究中，抗WA Id将用于模型系统。 在 SLE的主要重点将是研究血清低 分子量Clq反应物，以确定它们与 相关的低补体血症和抗体，以天然 患者体内的DNA与这些反应物发生反应。 新的尝试 将开发一种抗天然DNA XId抗体， 仅与nDNA抗体反应。 这种试剂将 这对这些研究很有帮助。

Previous work on the role of immune complexes in the pathogenesis of systemic lupus erythematosus (SLE), rhematoid arthritis and related disorders will be continued. In studies on RA, mixed connective tissue disease and essential mixed cryoglobulinemia (EMC), the role of rheumatoid factors with BLA and WA cross idiotypes (XIds) will be investigated. Polyclonal RFs bearing the BLA XId appear to occur in high incidence in rheumatoid arthritis while those bearing the WA XId occur widely in EMC and primary Sjogren syndrome. The BLA group of RFs are unique in reacting with DNA nucleoprotein in addition to IgG. In RA emphasis will be placed on characterization of immune complexes containing BLA RFs. Using sensitive immunoblotting techniques non-IgG antigen cross reactive with RF and antiidiotype antibodies will be sought. Similar studies will be done on the cryoglobulin from patients with EMC and the hypothesis will be tested that antiidiotype antibody is involved in the pattern of early complement component activation seen in this disease. Newly developed mouse monoclonal anti WA XId and anti WA Id will be used on a model system in these studies. In SLE the primary emphasis will be on studying the serum low molecular weight Clq reactants to determine their relationship to the associated hypocomplementemia and antibodies to native DNA that occurs in patients with these reactants. New attempts will be made to develop an anti native DNA XId antibody that reacts only with nDNA antibodies. Such a reagent would markedly facilitate these studies.