RESPONSE OF TUMOR CELLS TO HEAVY IONS

这项研究的目的是确定 影响肿瘤对稀疏性和非特异性免疫反应的多种细胞过程 高电离辐射。这是通过使用 具有良好特性的动物肿瘤系统(WAG/Rij中的R-1肉瘤 鼠)。重带电粒子辐射的响应可在 伯克利·贝瓦拉克与225千伏X射线的反应进行了比较。 这些研究的目的是提供关于 放射肿瘤中的下列细胞过程：再繁殖、修复 潜在致命性损伤、细胞时相特定存活率和 复氧作用。通过测量以下物质的比例来研究种群再繁殖 肿瘤切除实验中克隆形成细胞与时间的关系。修补 通过添加药物来衡量潜在致命损害的程度 照射后一系列时间的β-Ara-A。细胞特定的阶段 通过在肿瘤后引入Hoescht染色33342来衡量存活率 将细胞群切除并分选为G1、S和G2+M组分 FACS流式细胞仪。通过切除试验测量复氧量。 方法。 收集的数据将被用来进一步开发细胞动力学模型 高LET和低LET照射后肿瘤体积反应。 这个项目将提供对相关人员更多的了解 这些过程在肿瘤反应中的重要性。这一信息将是 对目前正在进行的重电荷粒子放射治疗计划有用 是在贝瓦拉克重离子加速器进行的。

The objective of this research is to determine the relative importance of various cellular processes in affecting tumor response to both sparsely and highly ionizing radiation. This is accomplished by using a well-characterized animal tumor system (the R-1 sarcoma in the WAG/Rij rat). The response from heavy charged particle radiation available at the Berkeley Bevalac is compared with the response from 225-kVp X rays. The studies are designed to provide information on the time course of the following cellular processes in irradiated tumors: repopulation, repair of potentially lethal damage, cell phase-specific survival and rates of reoxygenation. Repopulation is studied by measuring the fraction of clonogenic cells versus time by means of the tumor excision assay. Repair of potentially lethal damage is measured by the addition of the drug Beta-ara-A at a series of times postirradiation. Cell phase-specific survival is measured by introducing the Hoescht stain 33342 after tumor excision and sorting the cell population into G1, S and G2+M fractions with a FACS flow cytometer. Reoxygenation is measured via the excision assay method. The data gathered will be used to further develop a cell-kinetic model of tumor volume response after irradiation with high- and low-LET radiation. This project will provide an increased understanding of the relative importance of these processes in tumor response. This information will be useful to the heavy-charged particle radiotherapy program presently being conducted at the Bevalac heavy-ion accelerator.