DIFFERENTIATION OF CAPILLARY ENDOTHELIAL CELLS

实验的目的是：(1)建立原代培养的内皮细胞 来源于选定的胚胎雏形、胎儿器官和 选定的成人来源；(2)生产和鉴定这些来源的标记试剂 内皮细胞帮助检测和鉴定血管内皮细胞 一般和组织或器官定义的毛细血管内皮细胞亚群 更具体地说；(3)分析了肿瘤诱发和转移的过程 淋巴细胞诱导的血管生成，因为这可能是不同的反映或 共同为毛细血管内皮细胞来源的各种 来源；(4)启动旨在确定角色的发展研究 内皮细胞在选择性细胞黏附中的作用；(5)启动 小鼠毛细血管形态发生的发育分析 小鼠胚胎发生。 希望概述的研究将有助于更好地理解 发展中的血管系统，更具体地说，它将 提供新的洞察，了解伴随 肿瘤的发展和转移扩散，这是必须的 与炎症过程有关。(M)

Experiments are designed to: (1)\establish primary cultures of endothelium derived from capillaries of selected embryonic rudiments, fetal organs, and selected adult sources; (2)\produce and identify marker reagents for these endothelial cells to help detect and characterize endothelial cells in general and tissue or organ-defined capillary endothelial subpopulations more specifically; (3)\analyze the process of tumor-induced and lymphocyte-induced angiogenesis as this may be reflected differentially or in common for the capillary endothelial cells derived from the various sources; (4)\initiate developmental studies aimed at determining the role of endothelial cells in selective cell adhesion; and (5)\initiate developmental analysis of the morphogenesis of mouse capillaries during mouse embryogenesis. It is hoped that the outlined research will lead to a better understanding of the developing vascular system and, more specifically, that it will provide new insight into the neovascular process that accompanies the development and metastatic spread of tumors and that is in obligatory association with the inflammatory process. (M)