DIFFERENTIATION OF CAPILLARY ENDOTHELIAL CELLS

本实验的目的是：（1）建立内皮细胞原代培养体系 来源于选定的胚胎雏形、胎儿器官和 选择成人来源;（2）\生产和识别这些标记试剂 内皮细胞，以帮助检测和表征 一般和组织或器官定义的毛细血管内皮亚群 （3）分析肿瘤诱导的过程， 淋巴细胞诱导的血管生成，因为这可能反映差异或 在共同的毛细血管内皮细胞来源于各种 （4）开展旨在确定其作用的发展研究 内皮细胞在选择性细胞粘附;和（5）\启动 小鼠毛细血管形态发生的发育分析 小鼠胚胎发生 希望概述的研究将导致更好地了解 血管系统的发育，更具体地说， 提供了新的见解新生血管的过程，伴随着 肿瘤的发展和转移扩散，这是在强制性的 与炎症过程有关。 （百万）

Experiments are designed to: (1)\establish primary cultures of endothelium derived from capillaries of selected embryonic rudiments, fetal organs, and selected adult sources; (2)\produce and identify marker reagents for these endothelial cells to help detect and characterize endothelial cells in general and tissue or organ-defined capillary endothelial subpopulations more specifically; (3)\analyze the process of tumor-induced and lymphocyte-induced angiogenesis as this may be reflected differentially or in common for the capillary endothelial cells derived from the various sources; (4)\initiate developmental studies aimed at determining the role of endothelial cells in selective cell adhesion; and (5)\initiate developmental analysis of the morphogenesis of mouse capillaries during mouse embryogenesis. It is hoped that the outlined research will lead to a better understanding of the developing vascular system and, more specifically, that it will provide new insight into the neovascular process that accompanies the development and metastatic spread of tumors and that is in obligatory association with the inflammatory process. (M)