THE EXPRESSION OF ONCOGENICITY OF SHOPE FIBROMA VIRUS

这项建议的主要目标是确定Shope纤维瘤的作用 病毒(SFV)在病原中的异常生长。为此，有两个品系 在细胞培养中表现完全不同的SFV，一种促进形成的 一种是“病灶”，另一种是引起快速细胞溶解，将会被研究。 比较研究包括宿主DNA合成的测定， 病毒诱导蛋白和结构蛋白的特性及其对蛋白质的分析 用限制性内切酶分析病毒DNA。 将检查“焦点”中细胞的属性以确定是否 它们已经被永久性地修改了。此外，一年级的改造 将尝试用SFV进行细胞培养。在这些研究中，病毒基因组 将被灭活和/或细胞将被肿瘤促进剂和 化学致癌物。 从拟议工作中寻求的信息可能对以下方面具有潜在重要性 解释病毒致癌和化学致癌的分子基础。

The main objective of this proposal is to establish the role of Shope fibroma virus (SFV) in the genesis of abnormal growth. For this purpose two strains of SFV that behave entirely different in cell cultures, one promoting the formation of "foci" and the other inducing rapid cell lysis, will be investigated. Comparative studies include determination of host DNA synthesis, characterization of virus-induced and structural proteins and analysis of the viral DNA by restriction endonucleases. The properties of the cells in the "foci" will be examined to determine whether they have been permanently modified. In addition, transformation of primary cell cultures with SFV will be attempted. For these studies the viral genome will be inactivated and/or the cells will be pretreated with tumor promoters and chemical carcinogens. Information sought from the proposed work can be of potential importance to explain the molecular basis of both viral and chemical carcinogenesis.