Elucidation of DNA replication, distribution and maintenance mechanisms of oncogenic virus by using cellular factors

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Latency-associated nuclear antigen (LANA) recruits cellular DNA replication factors to the DNA origin (ori-P) in Kaposi's sarcoma-associated herpesvirus. To investigate the functional significance of the nuclear matrix in viral DNA replication, we constructed the chimeric protein of DBD fused to NuMA, which is one of the nuclear matrix proteins, and tested its DNA replication activity. Here we demonstrated that the NuMA-DBD localized in the nuclear matrix fraction, and it bound to the ori-P region, and showed high replication activity, which is similar to the full length of LANA. These results suggest that the recruitment of ori-P to the nuclear matrix is important for viral DNA replication.