PRESURGICAL INTRAVENOUS NUTRITION AND RECTAL CANCER

对动物肿瘤模型的研究表明，肿瘤的生长速度加快。 通过营养养生来补充营养不良的宿主。然而， 基于x光和体检的临床观察没有 检测到肿瘤生长的变化。这些检测肿瘤的方法 生长对分子上肿瘤生长的细微变化不敏感 水平。这项建议的目的是评估以下各项的效果 术前静脉高营养对肿瘤生长的影响 细胞动力学分析测得的参数。患者有一种 活检证实为直肠癌，需要至少七天的 术前IVH将符合这项研究的条件。直肠癌一直是 之所以选择进行这项研究，是因为足够的肿瘤组织采样可以 通过直肠镜可以很容易地获得。直肠肿瘤活组织检查将 在IVH开始前、IVH期间和完成时获得 手术前IVH就在手术切除前。所有肿瘤 采样将由直肠镜完成，以确保一致性 组织取样的条件。肿瘤组织和邻近正常直肠组织 将分析粘膜生长分数、S期细胞百分比和 用流式细胞仪检测DNA、RNA和蛋白质含量。首字母 肿瘤细胞动力学参数将与随后的 每个肿瘤的动力学参数。

Studies with animal-tumor models have shown that tumor growth is enhanced by nutritional regimens that replenish the malnourished host. However, clinical observations based upon x-rays and physical examination have not detected changes in tumor growth. These methods for detecting tumor growth are insensitive to subtle changes in tumor growth on the molecular level. The purpose of this proposal is to evaluate the effects of preoperative intravenous hyperalimentation (IVH) on tumor growth parameters as measured by cell kinetic analysis. Patients who have a biopsy proven rectal cancer and who require at least seven days of preoperative IVH will be eligible for this study. Rectal cancer has been selected for this study because adequate tissue sampling of the tumor can be easily obtained through a proctoscope. Rectal tumor biopsies will be obtained before IVH is started, during IVH and at the completion of preoperative IVH just before the surgical resection is done. All tumor sampling will be accomplished by the proctoscope to insure uniform conditions for tissue sampling. Tumor tissue and adjacent normal rectal mucosa will be analyzed for growth fraction, percent cells in S-phase and DNA, RNA and protein content as measured by flow cytometry. The initial tumor cell kinetic parameters will be compared with the subsequent kinetics parameters for each tumor.