13TSB_SynBio - Rapid Engineering of Cellular Factories

这是一个合作研发项目，目的是展示细菌细胞工厂的快速创建。在未来的10-20年里，全球化学应用工业预计将经历一场重大变革。由于需要开发经济和环境可持续的工艺，该行业将越来越多地转向可再生原料，以制造越来越多的产品。据估计，到2020年，大约20%的现有化学过程将基于工业生物技术（IB），先进的合成生物学和生物过程。在这个项目中，我们打算联合收割机几种新技术，包括生物泵，核糖开关和生物过程优化，以创造能够选择性地导入小分子的工程微生物，通过短合成途径转化它们，并以整个细胞为基础输出完成的分子。这种方法有可能彻底改变生物催化和IB，并且另外限制了技术风险，因为生物泵的使用仅允许优化短合成途径，而不是利用来自基本原料的生物体的天然代谢途径。这笔赠款的产出将导致替代的可持续化学生产工艺，有可能减少对石化原料的依赖。该研究项目还将产生技术和知识，这将有助于保持英国的竞争优势，并将产生训练有素和熟练的研究人员。

This is a collaborative R&D project, with the goal of demonstrating the rapid creation of bacterial cellular factories. Over thenext 10-20 years the global chemistry using industry is predicted to undergo a major transformation. Driven by the need todevelop processes that are both economically and environmentally sustainable, the industry will increasingly turn torenewable feedstocks for the manufacture of a growing range of products. It is estimated that by 2020 approximately 20%of existing chemical processes will be based on Industrial Biotechnology (IB), and advanced synthetic biology andbioprocesses.Within this project we intend to combine several novel technologies, including biopumps, riboswitches, and statisticalprocess optimization to create engineered micro-organisms which are able to selectively import small molecules, convertthem via short synthetic pathways, and export the finished molecules on a whole cell basis. This methodology has thepotential to revolutionize biocatalysis and IB, and additionally constrains the technical risk, as the use of biopumps allowsthe optimization of only short synthetic pathways, rather than utilizing an organism's native metabolic pathways from basicfeedstocks. The outputs from this grant will lead to alternative sustainable chemical production process, with the potentialto reduce the reliance on petrochemically derived feedstocks. This research project will also generate technology andknowledge that will help maintain the UK's competitive edge, and will produce highly trained and skilled researchpersonnel.