IN VITRO ANALYSIS OF ANTIBODY REGULATION IN HUMANS

人淋巴母细胞样B细胞是高度活化的B细胞的亚群，其 通过体内免疫诱导。 在体外，这些B细胞产生 不需要T细胞帮助或促分裂原刺激， 大多数抗体产生在培养的第3天完成。 在 在过去的一年里，我们发现这些细胞产生抗体的能力 可以通过NK细胞和单独的抑制性T细胞在体外降低 子集 在接下来的一年里，我们将确定是否所有的NK细胞都有 这种抑制能力，或者正如我们早期的研究所表明的， NK细胞的亚群执行该功能。 我们将通过选择性的 使用单克隆抗体去除和/或分离NK细胞的不同亚群 不同特异性的抗体。 我们最近已经确定，这两种抑制细胞识别 B细胞上的不同靶结构，NK细胞识别 转铁蛋白受体和T细胞识别一个单独的， 未定义的结构 从我们的研究中也清楚地看出，并非所有的淋巴母细胞样B细胞都是 能够被抑制。 尽管这两种抑制细胞 不同的目标结构，它们似乎识别相同的B细胞， 子集 这个B细胞阶段似乎是一个增殖细胞， 还没有发展出分泌抗体的能力。 （LB）

Human lymphoblastoid B cells are a subset of highly activated B cells which are induced by in vivo immunization. In vitro these B cells produce antibody without the need for T-cell help or mitogen stimulation, and the majority of the antibody production is complete by day 3 of culture. In the past year, we found that the antibody-producing capacity of these cells can be reduced in vitro by NK cells and a separate suppressor T-cell subset. In the coming year, we will determine whether all NK cells have this inhibitory capacity or, as suggested by our earlier studies, only a subset of NK cells performs this function. We will test this by selective removal and/or isolation of different subsets of NK cells using monoclonal antibodies with differing specificities. We have recently determined that the two suppressor cells recognize different target structures on the B cell, with the NK cell recognizing the transferrin receptor and the T cell recognizing a separate, as yet undefined structure. It is also clear from our studies that not all lymphoblastoid B cells are capable of being inhibited. Although the two suppressor cells recognize different target structures, they appear to recognize the same B-cell subsets. This B-cell stage appears to be a proliferating cell which has not yet developed the capacity for antibody secretion. (LB)