HUMAN PAPILLOMAVIRUSES IN GENITAL TRACT DYSPLASIAS

这项研究的长期目标是为 了解人乳头瘤病毒在人类乳头状瘤发生发展中的作用 宫颈不典型增生和浸润性癌。具体目标包括： 1)建立HPV类型DNA倍体模式与 组织切片中可见的形态计量学参数。 2)a)表征病毒DNA的状态。 B)绘制病毒转录本地图。 C)鉴定“早期”病毒蛋白并阐明其在宫颈中的功能 癌症和肿瘤来源的细胞系。 3)研究乳头瘤病毒感染时的免疫反应。 A)确定是否存在针对“早期”病毒蛋白的抗体。 B)尝试为“致癌的”人乳头瘤病毒“S”构建型特异性抗原 在人类血清中寻找这些抗原的抗体，可能会提供一种 关于这些病毒在人类群体中传播的迹象。

The long term goals of this research are to provide evidence for and an understanding of the role of human papilloma viruses in the development of cervical dysplasias and invasive carcinomas. The specific aims include: 1) Establish a correlation between HPV types DNA ploidy patterns and morphometric parameters seen in histologic sections. 2) a) Characterize the state of viral DNA. b) Map viral transcripts. c) Identify "early" viral proteins and elucidate their function in cervical carcinomas and carcinoma derived cell lines. 3) Investigate aspects of the immune response in papillomavirus infections. a) Determine the presence of antibodies to "early" viral proteins. b) Attempt to construct type specific antigens for "oncogenic" HPV"s and search for antibodies to these antigens in human sera which may provide an indication about the spread of these viruses in the human population.