NMR STUDIES OF HETEROGENEITY & RADIORESISTANCE OF TUMORS

体内磁共振波谱（MRS）/成像（MRI）最近已经 被广泛用于研究生理和代谢产物 鼠和人肿瘤的改变。结果发现，31 P 和1H MRS在肿瘤膜代谢和生物能量学方面提供信息， 并可能成为肿瘤的临床监测和/或预测因子 对治疗的反应。然而，由于其多样性和复杂性， 肿瘤，从传统的肿瘤中理解这些变化， 生物化学观点普遍较差， needed. 对植物的生理和生化进行了广泛的相关研究 肿瘤特性及其对辐射反应的改变 提出了在原位和体外使用磷和氢MRS。在 简要：（a）MRS观察到的代谢物和pH值的变化， 具有不同的宿主/肿瘤细胞比率，Q/P细胞， 缺氧分数和细胞周期时相分布将被分析， 相关;（B）亚群对固有辐射抗性的影响 将使用MRS进行检查，以阐明生物因素 引起代谢物辐射诱导的改变;（c） 最终，体内MRS参数作为临床监测器的意义 和/或将推导出预测器。

In vivo magnetic resonance spectroscopy (MRS)/imaging (MRI) has recently been extensively used in investigating the physiology and metabolite alterations of both murine and human neoplasms. It was found that the 31P and 1H MRS are informative in tumor membrane metabolism and bioenergetics, and could potentially be the clinical monitor and/or predictor of tumor response to treatments. However, due to the diversity and complexity of the tumors, the understanding of these alterations from the conventional tumor biochemistry points of view is generally poor and immediate exploration is needed. An extensive correlative study into the physiology and biochemistry of tumor characteristics and their alterations in response to irradiation using both phosphorous and hydrogen MRS in situ and vitro is proposed. In brief: (a) The variation of MRS-observed metabolites and pH of various subpopulations with different ratios of host/tumor cells, Q/P cells, hypoxic fractions, and cell cycle phase distributions will be analyzed and correlated; (b) The effects of subpopulation on intrinsic radioresistance will be examined using MRS in order to elucidate the biological factors which cause the radiation-induced alteration in the metabolites; (c) Ultimately, the significance of in vivo MRS parameters as clinical monitors and/or predictors will be deduced.