QUANTITATION OF LYMPH NODE DISEASES

组织活检的显微镜检查是检查的“黄金标准” 这些诊断是在外科病理学中提出的。这项检查是在 通过光学显微镜使用几个级别的放大。人类 眼睛，尽管这是一个从 组织切片的图像和确定广泛的类别 疾病，不产生对组织特性的定量描述。在……里面 我们之前的研究发现，计算机化(机器人)显微镜是 能够在很大程度上帮助病理学家形成诊断 在滤泡性淋巴瘤的亚型方面，来自数字显微照片。 这一点很重要，因为患者的治疗计划取决于关键 基于子类型确定的准确性。适当的治疗， 当然，反过来又对患者的生活质量有重大影响 以及生存时间。 到目前为止，我们的工作主要集中在中等分辨率的计算机化上 滤泡中心细胞显微镜检查(每例约20,000例) 覆盖一或两平方毫米的面积。输入小的， 混合和大型细胞类别的表现不亚于或更好 机器人显微镜比一个专家病理专家小组所做的要好。在……里面 拟议的研究，我们打算将这项工作扩大到涵盖更大的范围 组织面积(一到两平方厘米)，包括几个 总共有百万个细胞。这样做的目的是量化低- 与许多良性疾病相区别的解决模式 恶性肿瘤。特别是，这项研究将解决以下问题 颜色量化，这在我们以前的工作中没有解决。这个 使用的机器人显微镜具有自动颜色切换功能，覆盖550 nm，620 Nm和450 nm。在过去，颜色角度测量被发现是 有助于区分存在于 慢性活动性肝炎和慢性迁延性肝炎。在建议的 研究这些颜色角度测量将尽可能地进行调查 不仅用于区分卵泡低分辨率模式的工具 淋巴瘤也是弥漫性淋巴瘤的细胞亚型。一些人 按照这些思路进行的初步工作如图所示，似乎 很有希望。

Microscopic examination of the tissue biopsy is the "gold standard" upon which diagnoses is rendered in surgical pathology. This examination is made via the optical microscope using several levels of magnification. The human eye, although a remarkable instrument for extracting the "gestalt" from imagery of the tissue sections and determining the broad category of disease, does not yield a quantitative description of tissue properties. In previous research we have found that the computerized (robot) microscope is capable of assisting the pathologist significantly in forming determinations from digital micrographs as regards the subtyping of follicular lymphoma. This is significant in that the patient treatment plan depends critically upon the accuracy of the subtype determination. Proper treatment, of course, in turn has a major influence on both the patient's quality of life and length of survival. Our work to date has concentrated on moderate resolution computerized microscopy of follicular center cells (approximately 20,000 per case) covering an area of one or two square millimeters. Typing into small, mixed, and large cell categories has been performed as well as or better by the robot microscope than that done by a panel of expert pathologists. In the proposed research, we intend to expand this work to cover much larger areas of tissue (one to two square centimeters) which would encompass a few million cells in toto. The purpose of so doing is to quantitate the low- resolution pattern from which many benign diseases are differentiated from malignancies. In particular, the research will address the question of color quantitation which has not been addressed in our previous work. The robot microscope employed has automated color switching to cover 550 nm, 620 nm, and 450 nm. In the past, color angle measurements have been found to be useful in distinguishing between the subtle differences which exist in chronic active hepatitis and chronic persistent hepatitis. In the proposed research these color angle measurements will be investigated as possible tools for distinguishing not only low-resolution patterns in follicular lymphomas but also the cellular subtype in diffuse lymphomas. Some preliminary work along these lines is illustrated and appears to be promising.