PROGESTERONE RECEPTOR REGULATION IN ENDOMETRIAL CANCER

一种人子宫内膜癌的实验模型已经建立 人的肿瘤在其中生长并裸体传代 维持其生化和形态的小鼠 特点。孕激素受体已被鉴定为 并从裸鼠体内生长的肿瘤中部分提纯，以及 已经制备出了单抗。这些实验性的 工具，以及灵敏地调节孕酮的能力 这个实验系统中的受体使我们处于理想的位置 对纯化产物的理化性质进行了研究 黄体酮受体蛋白，它反过来将作为 与这些改变后的特征进行比较的基础 体内孕激素受体调控中的蛋白质。 因此，这项建议的具体目的是：1)研究 人子宫内膜癌PR的结构研究 调整公关结构，3)确定关系 PR结构与生物反应之间的关系；4)分析 孕激素下调PR的机制。这些研究 将极大地促进我们对生物的理解 孕激素在子宫内膜癌中的长期作用 这项提案的目标。

An experimental model of human endometrial carcinoma has been developed, in which human tumors are grown and passaged in nude mice with maintenance of their biochemical and morphological characteristics. The progesterone receptor has been characterized and partially purified from tumors grown in nude mice, and monoclonal antibodies have been prepared. These experimental tools, and the ability to sensitively modulate the progesterone receptor in this experimental system place us in an ideal position to study the physicochemical characteristics of purified progesterone receptor proteins, which in turn, will serve as the basis for comparison with the altered characteristics of these proteins during the regulation of progesterone receptor in vivo. Therefore, the specific aims of this proposal are 1) to study the structure of human endometrial carcinoma PR 2) to investigate the regulation of PR structure, 3) to determine the relationship between PR structure and biological response and 4) to analyze the mechanism of down regulation of PR by progestins. These studies will significantly advance our understanding of the biology of progestin action in endometrial carcinoma which is the longterm objective of this proposal.