RELATIONSHIP BETWEEN BLOOD AND TISSUE MICRONUTRIENT

累积的流行病学和实验室研究结果表明， 某些微量营养素可抑制或防止致癌过程。 由于这些微量营养素可以在特定组织发挥作用， 适当的目标组织浓度的微量营养素， 对于预防癌症和/或癌前病症至关重要。 在 为了确定微量营养素在癌症预防中的作用， 因此，重要的是研究各种组织的微量营养素含量 在人类中，特别是那些正常的，癌前病变和癌症。 在拟议的研究中，我们将确定各种 微量营养素，包括维生素A、β-胡萝卜素、番茄红素和维生素 E，在正常人的血浆、口腔粘膜细胞、皮肤和宫颈组织中 个体、高危受试者和癌症患者。 我们还将 比较正常人、 同一癌症患者的癌前组织和癌组织， 癌症患者的癌组织和 匹配的对照。 因为除了血液和口腔粘膜以外的组织 细胞很难在人类中获得，因此，我们将研究 血浆和/或口腔粘膜细胞的微量营养素含量是否可以 作为一项指标，用于评估 问题. 这将通过调查 这两种组织中的微量营养素含量 目标组织如皮肤和子宫颈的微量营养素含量， 癌症患者和非癌症个体。 的分析方法 上述微量营养素的分析在我们的 实验室 广泛的质量控制/质量保证（QC/QA）计划 将遵循，以确保分析数据的准确性。 我们的实验室一直被评为最准确的 分析实验室之间的参与者在国家统计局 标准赞助的QC/QA程序，用于分析维生素A、β-胡萝卜素、 维生素E自1984 我们亚利桑那大学癌症研究小组 中心是多学科的，并有必要的专业知识，以进行 建议的研究。 我们计划在四年内完成调查。

The accumulated epidemiologic and laboratory findings have suggested that some micronutrients may inhibit or prevent the process of carcinogenesis. Since these micronutrients may exert their actions at specific tissue sites, adequate target tissue concentration of the micronutrient may be critical for the prevention of cancer and/or precancerous conditions. In order to define the role of micronutrients in cancer prevention, it is, therefore, important to study the micronutrient content of various tissues in humans, especially those with normal, premalignant lesions, and cancers. In the proposed study, we will determine the content of various micronutrients, including vitamin A, beta-carotene, lycopene, and vitamin E, in plasma, buccal mucosal cells, skin, and cervical tissues of normal individuals, subjects at high risk, and cancer patients. We will also compare the difference in micronutrient content between normal, precancerous, and cancerous tissues of same cancer patient as well as between cancerous tissues from cancer patients and normal tissues from matched controls. Because tissues other than blood and buccal mucosal cells are difficult to obtain in humans, we will, therefore, investigate whether the micronutrient content of plasma and/or buccal mucosal cells can be used as an indicator for assessing the micronutrient status of other issues. This will be accomplished by investigating the relationship between the micronutrient content of these two tissues and the micronutrient content of the target tissues such as skin and cervix in cancer patients and non-cancer individuals. The analytical procedures for analysis of the above micronutrients are well established in our laboratory. An extensive quality control/quality assurance (QC/QA) program will be followed in order to assure the accuracy of the analytical data. Our laboratory has been consistently rated as one of the most accurate analytical laboratories among the participants in the National Bureau of Standards sponsored QC/QA program for analysis of vitamin A, beta-carotene, and vitamin E since 1984. Our group at the University of Arizona Cancer Center is multidisplinary and has the necessary expertise to conduct the proposed study. We plan to complete the investigations in 4 years.