CORE--ANALYTICAL CHEMISTRY

分析化学中心，在彭亦梅博士的监督下， 将继续为分析化学领域提供支持和服务 对所有项目提出建议。 这件事的主要责任 核心仍然是对提交的生物样本进行分析， 为调查人员提供准确和及时的方式。 具体分析 由本核心提供的将包括：视黄醇，棕榈酸视黄酯，β- 胡萝卜素、α-生育酚、α-生育酚乙酸酯、α-生育酚 琥珀酸盐，二氟甲基鸟氨酸（DfMO），烟酰胺，单宁酸， 多胺、前列腺素E2 D、BrdU和PCNA标记指数，以及突变体 Ha-ras基因和p53基因拷贝数。 此外，核心将 开发新化合物的分析方法，并进行 化合物的化学性质和稳定性研究 确保生物样品的适当处理和储存 在分析之前。 为了确保我们的信息的准确性和一致性， 分析数据，核心将继续遵循现有的质量 我们开发的控制/质量保证计划以及 美国国家标准与技术研究院 脂肪酸分析核心，在唐纳德博士的监督下 McNamara将在脂肪酸领域提供服务和支持 项目三分析。细胞荧光造影核心， Raymond Taetle博士的监督，将进行检测，以监测 外周血淋巴细胞亚群用于项目IIC和IVA。 该核心还将建议项目III用于检测细胞 黑素细胞表面标记物和用于检测T- 回到皮肤的细胞。

The Analytical Chemistry Core, under the supervision of Dr. Yei-Mei Peng, will continue to provide support and service In the analytical chemistry area to all the proposed projects. The primary responsibility of this Core continues to be the analysis of the submitted biological samples in an accurate and timely manner for the investigators. The specific assays provided by this Core will Include: retinol, retinyl palmitate, beta- carotene, alpha-tocopherol, alpha-tocopheryl acetate, alpha-tocopheryl succinate, difluoromethylornithine (DfMO), nicotinamide, tannic acid, polyamines, prostaglandin E2D BrdU and PCNA labeling index, and mutant Ha-ras gene and p53 gene copy numbers. In addition, the Core will develop methods for the analysis of new compounds and also conduct research on the chemical properties and stability of the compounds of interest to ensure the proper handling and storage of biological samples prior to analysis. To assure the accuracy and consistency of our analytical data, the Core will continue to follow the existing quality control/quality assurance program we developed as well as the program of the National Institute of Standards and Technology. The fatty Acid Analysis Core, under the supervision of Dr. Donald McNamara, will provide service and support In the area of fatty acid analysis for project III. The Cytofluorography Core, under the supervision of Dr. Raymond Taetle, will perform assays to monitor lymphocyte subpopulations in peripheral blood for projects IIC and IVA. This core will also advice project III for the detection of cell surface markers on melanocytes and project IVB for the detection of T- cells that home to the skin.