CORE--ANALYTICAL CHEMISTRY

分析化学核心，在彭业美博士的指导下， 将继续在分析化学领域提供支持和服务 所有拟议项目的面积。这件事的主要责任是 核心继续对#年提交的生物样本进行分析 为调查人员提供准确和及时的信息。具体的分析方法 由此核心提供的将包括：视黄醇，视黄醇棕榈酸酯，贝塔- 胡萝卜素、α-生育酚、α-生育酚乙酸酯、α-生育酚 琥珀酸二氟甲基鸟氨酸(DFMO)，烟酰胺，单宁酸， 多胺、前列腺素E2D BrdU和增殖细胞核抗原标记指数及突变体 HA-ras基因和P53基因拷贝数。此外，核心将 开发新化合物的分析方法，并进行 黄曲霉毒素类化合物的化学性质及稳定性研究 确保适当处理和储存生物样本的利益 在分析之前。确保我们的数据的准确性和一致性 分析数据，核心将继续遵循现有的质量 我们开发的控制/质量保证计划以及 美国国家标准与技术研究所。 在Donald博士的监督下，脂肪酸分析核心 McNamara将在脂肪酸领域提供服务和支持 对项目三.细胞荧光照相核心的分析 Raymond Taetle博士的监督，将进行化验以监测 IIC和IVA项目外周血中的淋巴细胞亚群。 该核心还将为项目III提供细胞检测方面的建议 黑素细胞表面标志物和IVB投影检测T细胞 这些细胞是皮肤的家园。

The Analytical Chemistry Core, under the supervision of Dr. Yei-Mei Peng, will continue to provide support and service In the analytical chemistry area to all the proposed projects. The primary responsibility of this Core continues to be the analysis of the submitted biological samples in an accurate and timely manner for the investigators. The specific assays provided by this Core will Include: retinol, retinyl palmitate, beta- carotene, alpha-tocopherol, alpha-tocopheryl acetate, alpha-tocopheryl succinate, difluoromethylornithine (DfMO), nicotinamide, tannic acid, polyamines, prostaglandin E2D BrdU and PCNA labeling index, and mutant Ha-ras gene and p53 gene copy numbers. In addition, the Core will develop methods for the analysis of new compounds and also conduct research on the chemical properties and stability of the compounds of interest to ensure the proper handling and storage of biological samples prior to analysis. To assure the accuracy and consistency of our analytical data, the Core will continue to follow the existing quality control/quality assurance program we developed as well as the program of the National Institute of Standards and Technology. The fatty Acid Analysis Core, under the supervision of Dr. Donald McNamara, will provide service and support In the area of fatty acid analysis for project III. The Cytofluorography Core, under the supervision of Dr. Raymond Taetle, will perform assays to monitor lymphocyte subpopulations in peripheral blood for projects IIC and IVA. This core will also advice project III for the detection of cell surface markers on melanocytes and project IVB for the detection of T- cells that home to the skin.