ANTITUMOR, ANTIVIRAL AGENTS IN MICROALGAE

这项建议是为了研究微藻，特别是甲藻， 用于新型抗肿瘤、抗病毒和抗微生物药物。这项研究是 也意在确定这些微藻是否是 最近在海洋中发现的高度活跃的有趣分子 无脊椎动物，因此可以成为潜在的可靠来源 重要的治疗药物。预期的化合物包括聚醚， 大环内酯类化合物和其他多氧化合物。该提案的基础是 这些化合物的最新发现在 甲藻。 具体地说，自由生活和共生或相关的有机体都将 被隔离并大规模培养。重点将放在新鲜的 来自自然栖息地或宿主的分离菌株，尽管那些保存在 文化收藏和我们已经拥有的也将是 调查过了。细胞和肉汤将被提取，提取液 将由生物检测指导进行分级。纯化产物的结构 化合物将通过物理化学方法进行研究，包括高 场核磁共振、质谱学和X射线结晶学。抗肿瘤和 抗病毒检测将由百时美施贵宝公司和NCI进行，并在- 室内细胞毒性测试将在有限的细胞系中进行， 常规隔离指南。抗菌测试将在室内进行。

This proposal is to investigate microalgae, especially dinoflagellates, for novel antitumor, antiviral and antimicrobial agents. The research is also intended to determine if these microalgae are the primary sources of highly active interesting molecules recently discovered in marine invertebrates, and thus can be reliable sources of these potentially important therapeutic agents. Anticipated compounds include polyethers, macrolides, and other polyoxygenated compounds. The proposal is based on the recent findings of the occurrence of these compounds in dinoflagellates. Specifically, both free-living and symbiotic or associated organisms will be isolated and cultured in large scale. Emphasis will be on freshly isolated strains from natural habitats or hosts, although those held in culture collections and already in our possession will be also investigated. The cells and broths will be extracted, and the extracts will be fractionated guided by bioassays. The structures of purified compounds will be studied by physico-chemical methods including high field NMR, mass spectroscopy, and X-ray crystallography. Antitumor and antiviral assays will be conducted by Bristol-Myers Co., and NCI, and in- house cytotoxicity tests will be performed with limited cell lines as routine isolation guides. Antimicrobial tests will be done in house.