ROLE OF DNA REPAIR IN SUSCEPTIBILITY TO SKIN CANCER

阳光中的紫外线辐射是导致皮肤癌的主要原因。 人类。然而，每个人的敏感度有很大的不同。 皮肤癌和其他器官的癌症。总目标 这个项目的关键是要了解决定 个人对皮肤癌的易感性。这一目标将在#年实现。 之前发现的一群马里兰州的船工，他们有很高的 非黑色素瘤皮肤癌(基底细胞癌和鳞状细胞癌 癌症)。对个人终生紫外线暴露的准确估计 是根据采访数据的组合为每个人计算的， 工作特点，以及现场和实验室测量。因此， 皮肤癌的主要危险因素，紫外线暴露，很容易 控制住了。影响皮肤风险的其他表型特征 癌症，如皮肤色素沉着、易晒伤(皮肤型)和头发 颜色，已为所有个人录制。 这项研究将检验DNA修复效率在易感性中的作用 非黑色素瘤皮肤癌。癌症患者的DNA修复效率和 配对的对照组将通过两种DNA修复分析进行评估：一种淋巴细胞- DNA光产物修复的质粒法和免疫测定法 在淋巴细胞和成纤维细胞中。其他变量，如寿命UV 曝光、日光敏感度和皮肤类型将在 分析。癌症发病年龄与DNA修复的关系 还将审查效率。 这项研究的结果应该有助于我们的整体理解 决定皮肤癌(和其他疾病)易感性的机制 癌症)在人类，II)帮助识别处于高位的个人 患皮肤癌的风险，以及iii)有助于估计 太阳紫外线潜在增加所产生的总体人口 地球表面。

Ultraviolet radiation in sunlight is the major cause of skin cancer in humans. However, individuals differ considerably in their susceptibility to cancer of the skin as well as cancer in other organs. The overall goal of this project is to understand the mechanisms involved in determining individual susceptibility to skin cancer. This goal will be addressed in a previously identified group of Maryland watermen who are at high risk for non-melanoma skin cancer (basal cell carcinoma and squamous cell carcinoma). Accurate estimates of individual lifetime UV exposures have been calculated for each individual from a combination of interview data, working characteristics, and field and laboratory measurements. therefore, the major risk factor for skin cancer, UV exposure, can be easily controlled. Other phenotypic characteristics which influence risk for skin cancer such as skin pigmentation, ease of sunburning (skin type), and hair color, have been recorded for all individuals. This study will examine the role of DNA repair efficiency in susceptibility to non-melanoma skin cancer. DNA repair efficiency of cancer cases and matched controls will be assessed by two DNA repair assays: a lymphocyte- plasmid reactivation assay and an immunoassay for DNA photoproduct repair in lymphocytes and fibroblasts. Other variables such as lifetime UV exposure, sun sensitivity and skin type will be controlled for in the analyses. Association between age at onset of cancer and DNA repair efficiency will also be examined. The results of this study should i) contribute to our overall understanding of the mechanisms that determine susceptibility to skin cancer (and other cancers) in man, ii) aid in the identification of individuals at elevated risk for skin cancer, and iii) be useful in estimating increased risks to the general population resulting from potential increases in solar UV at the earth's surface.