14IBCAT1FEASIBILITY Discovery and development of large/diverse user-friendly panels of novel biocatalysts

将开发生物化学试验，以促进对10类酶中每一类酶的成员进行初步筛选，筛选出这些酶可能转化的少量相关化合物。那些被证明转化一种或多种这样的化合物的酶将被详细地描述。这种分析将采用光吸收（分光光度法）、分离（色谱法）和质谱测定（质谱法）等技术。分析还将包括确定某些酶是否能够区分化合物的非重叠镜像（即立体异构体），因为这在为制药业生产纯化合物时至关重要。很可能有些酶不能转化所选的化合物。然后，这些酶将与其他化合物进行对比测试，这些化合物将通过研究相关科学文献获得信息。产生的结果将为生产酶的工业合作伙伴有效选择其他酶提供信息，这些酶与那些进行有趣转化的酶是互补的。这将增加产生的执行有趣转化的酶的总数，即产生广泛的筛选面板，因此将执行大范围的有趣转化。

Biochemical tests will be developed to facilitate initial screening of members from each of 10 classes of enzyme on a small range of relevant chemical compounds which the enzymes are likely to convert. Those enzymes that are shown to convert one or more such compounds will be characterized in detail. This analysis will employ techniques that involve absorbency of light (spectrophotometry), separation (chromatography) and mass determination (mass spectrometry). Analysis will also involve determining whether certain enzymes can distinguish between non-superimposable mirror images of chemical compounds (i.e. stereoisomers), as this is of critical importance when producing pure compounds for the pharmaceutical industry, for example. It is highly likely that some of the enzymes will not convert the chosen chemical compounds. These enzymes will then be tested against other chemical compounds that will be informed by studying the relevant scientific literature. The results generated will inform the efficient selection of other enzymes, by the industrial partner producing the enzymes, which are complementary to those that are performing interesting conversions. This will increase the total number of enzymes produced that perform interesting conversions, i.e. generate wide-ranging screening panels that will therefore perform a large range of interesting conversions.