SYNAPTIC TRANSMISSION DURING NEURONAL DIFFERENTIATION

描述：(改编自申请人的摘要。)宽泛的长期 目的是确定突触传递对神经的作用 差异化。调查人员将把重点放在主要细胞上 雏鸡的前庭切线核(哺乳动物外侧核) 前庭核团)。主要的前庭-眼反射。特价 将注意突触之间的传递方式。 发育中的主细胞和由巨型纤维形成的突触 前庭神经和巨大的纤维。在开发过程中， 巨大的纤维突触，或勺状末端，经历了几次转变 包括形成化学突触，然后是混合突触，最后 主要是与主细胞的缝隙连接。高度的 这种联系的特异性在脊椎动物的神经中是无与伦比的。 系统。调查人员将分析该病的发生和发展 没有详细说明的电传输，尽管它有 据报道，哺乳动物大脑中的几个部位发生了这种情况。 他们将利用脑片，因为切向核具有高度的 适合切片分析的有组织的结构。细胞内和 细胞外记录将应用于切向核 在刺激前庭神经节之后。膜和 主细胞的突触属性将在下列情况下定义 勺状突触表现出变化。它们将从形态上识别 用荧光染料和电子技术记录的细胞和突触 显微镜。先期缝隙连接的胚胎期分析 出现并将在其他关键年龄重复这一策略。 当中枢神经系统神经元之间的正常突触传递在 出生前或出生后早期发育，年轻人往往遭受减退 终生的智力能力，如学习能力 精神障碍和精神发育迟滞。这些关于发育中的神经元和 突触可能为预防策略或新的治疗方法提供基础 治疗突触传递疾病。此外，这些研究应该 有助于我们理解前庭系统是如何开始 功能及功能活动在神经元分化和分化中的作用 神经元存活。

DESCRIPTION: (Adapted from the Applicant's Abstract.) The broad long-term objective is to determine the role of synaptic transmission on neural differentiation. The investigators will focus on the principal cells of the tangential vestibular nucleus of the chick (mammalian lateral vestibular nucleus). The principal vestibulo-ocular reflexes. Special attention will be paid to the mode of synaptic transmission between the developing principal cells and the synapses formed by the giant fibers of the vestibular nerve, the colossal fibers. During development, the colossal fiber synapse, or spoon ending, undergoes several transitions including forming chemical synapses, then mixed synapses, and finally mainly gap junctions with the principal cells. The high degree of specificity in this connection is unsurpassed in the vertebrate nervous system. The investigators will analyze the onset and development of electrical transmission which has not been done in detail, in spite of its reported occurrence at several sites in the mammalian brain. They will utilize brain slices because the tangential nucleus has a highly organized structure well suited to slice analysis. Intracellular and extracellular recordings will be applied in the tangential nucleus following stimulation of the vestibular ganglion. The membrane and synaptic properties of the principal cells will be defined at times when the spoon synapses exhibit changes. They will identify morphologically the cells and synapses recorded from suing fluorescent dyes and electron microscopy. The analysis at the embryonic age when gap junctions first appear and will repeat the strategy at other critical ages. When normal synaptic transmission between CNS neurons is impaired during prenatal or early postnatal development, the young often suffer diminished intellectual abilities throughout life, as in the case of learning disorders and mental retardation. These studies on developing neurons and synapses may provide a basis for preventive strategies or new treatments for diseases of synaptic transmission. In addition, these studies should contribute to our understanding of how the vestibular system begins to function and the role of functional activity on neuron differentiation and neuron survival.