GLYCINE, SERINE AND THREONINE METABOLISM

这项研究的目的是刻画新陈代谢调节 哺乳动物肝组织中的丝氨酸、苏氨酸和甘氨酸 正常状态。将特别强调两国之间的关系 催化这些氨基酸分解代谢的关键步骤的酶 以及这些酶与不稳定甲基新生的关系 组。丝氨酸、苏氨酸和甘氨酸在哺乳动物肝脏中的代谢 组织将从互锁和相互作用的角度进行研究 推测在1-碳代谢中的作用。这些酶中有许多具有AS 辅助性因素：磷酸和/或叶酸。我们会研究 这些酶的催化机制。光谱研究和动力学 研究将使我们能够进一步描述这些反应。证据 关于这些酶的分布和划分，将 也被调查。氨基丙酮在代谢中的作用将是 调查过了。将特别关注如何解开 氨基酸的碳原子代谢率；甘氨酸、丝氨酸和 苏氨酸。确定用于研究的酶包括甘氨酸 裂解复合体、丝氨酸转羟甲基酶、氨基丙酮合成酶和 苏氨酸脱氢酶。将采取多方面的方法，这将 包括：有机合成、酶分离、动力学测量和 机械学研究。

The goal of this research is to characterize the metabolic regulation of serine, threonine and glycine in mammalian liver tissue and in diseased and normal states. Special emphasis will be placed on the relationship of the enzymes catalyzing committed steps in the catabolism of these amino acids and on the relationship of these enzymes to neogenesis of labile methyl groups. The metabolism of serine, threonine and glycine in mammalian liver tissue will be studied from the standpoint of their interlocking and putative roles on 1-carbon metabolism. Many of these enzymes have as co-factors pyridoxal phosphate and/or folic acid. We will study the mechanism of catalysis by these enzymes. Spectral studies and kinetic studies will enable us to further characterize these reactions. Evidence concerning the distribution and compartmentalization of these enzymes will also be investigated. The role of aminoacetone in metabolism will be investigated. Special attention will be directed towards unraveling the metabolic rate of the carbon atoms of the amino acids; glycine, serine and threonine. The enzymes identified for investigation include, glycine cleavage complex, serine transhydroxymethylase, aminoacetone synthase, and thronine dehydrogenase. A multi-faceted approach will be taken which will include: organic synthesis, enzyme isolation, kinetic measurements and mechanistic studies.