MASS SPECTROMETRY OF INVOLATILE BIOMOLECULES

质谱分析是一种必不可少的分析技术 重要的生物化合物。 因此， 质谱测定大的，不稳定的， 极性分子对化学研究的进展至关重要， 生物医学、生物化学和生物科学。 我们的总目标是扩大有机物质的效用 生物化学、生物医学和生物科学中的光谱学 一般来说，在促垂体激素的内分泌学研究中， 尤其是荷尔蒙。 我们的具体目标如下： 研究控制地球的物理和化学过程， 二次离子从液体和固体基质中的喷射， 受到粒子轰击的能量范围从 5-50 keV，以及ii）发展一种或多种能够 检测小于或等于1皮摩尔的单个寡肽 而不会不适当地牺牲质量的特异性 光谱法 当样品的质量下降时， 由于质量范围、灵敏度或 复合类型。 目前的质谱灵敏度是 特别是在研究中的限制，如对激素或 疾病的分子标记物，其中分析物的可用性 受到体内取样要求的严格限制。 在过去十年中发现的电离技术 大大增加了分子的种类和大小， 质谱分析;然而，已经注意到， 在最高的灵敏度，可电离的分子 在完全测试之前，需要称重（10，000 - 20，000道尔顿） 可以利用这些新的离子源。 在多大程度 其灵敏度可以增加而不会过度牺牲 定性的具体性是基础研究和应用研究的问题。 本提案中描述的调查方案不会 只是对我们理解 解吸电离过程，但它会利用这一宝贵的 大量的知识， 促垂体激素的光谱检测限， 这一成就将大大扩大 内分泌学研究。

Mass spectrometry is an indispensable technique for the analysis of biologically important compounds. Thus, the development of techniques for mass spectrometric determinations of large, labile, polar molecules is of great importance to progress in the biomedical, biochemical, and biological sciences. Our general objective is to expand the utility of organic mass spectrometry in biochemical, biomedical, and biological sciences generally and in endocrinological research on hypophysiotropic hormones particularly. Our specific aims are as follows: i) to investigate the physical and chemical processes that govern the ejection of secondary ions from liquid and solid matrices that are subjected to bomdardment by particles with energies ranging from 5-50 keV, and ii) to evolve one or more methods capable of detecting less than or equal to 1 pmole of individual oligopeptides without unduly sacrificing the qualitative specificity of the mass spectrometry. The usefulness of mass spectrometry is reduced when samples cannot be analyzed due to bounds on mass range, sensitivity, or compound types. Current mass spectrometric sensitivities are particularly restrictive in research, such as that on hormones or molecular markers for diseases, where the availability of analyte is severely limited by requirements for sampling in vivo. Ionization techniques discovered during the last decade have greatly increased the variety and size of molecules amenable to mass spectrometric analysis; it has been noted, however, that comparable gains in sensitivity at the highest, ionizable molecular weights (10,000-20,000 daltons) will be required before full advantage can be taken of these new ion sources. The extent to which sensitivity can be increased without unduly sacrificing qualitative specificity is a matter for basic and applied research. The program of investigation described in this proposal would not only make general contributions to our understanding of desorption ionization processes, but it would utilize that valuable body of knowledge to bring about a significant reduction in mass spectrometric detection limits for hypophysiotropic hormones, an accomplishment that would markedly expand the horizons of research in endocrinology.