ACID RAIN--AIR POLLUTANT INTERACTIONS

臭氧、酸雾和酸雨（包括硫酸气溶胶） 是主要的公共卫生问题，部分原因是 能够对肺部产生可测量的短暂影响， 易感受试者，浓度可能发生在 环境污染事件，部分原因是他们可能会采取行动， 协同作用对肺产生的影响大于 由个人曝光产生。 关于知识 臭氧-酸共生体对环境的可能有害影响 呼吸道仍然非常虚弱 绵羊模型 系统提出了检查之间的协同效应 臭氧和可吸入硫酸气溶胶。 在当前 我们将采用多学科方法， 评估臭氧、酸性气溶胶及其 联合用药，对呼吸道上皮和内皮细胞 磁导率 支气管肺泡清除率 将使用来自肺的放射性气溶胶来确定上皮细胞 渗透率和获得的结果将进行比较， 蛋白质、溶酶体酶和细胞的出现， 通过标准方法进行支气管肺泡灌洗。 取绵羊肺淋巴液， 99 mTc-DTPA经肺的渗透性和可能的清除率 反物质。 最后，在一个单独的系列中， 臭氧、酸气溶胶或组合臭氧-酸相同协议 气溶胶，气道血流将使用 多普勒流量计-遥测系统。 气道血液变化 流量预计将提供神经源性或介质的指标- 发生在气道炎症中的相关事件， 预期影响气道平滑肌以及气道 微脉管系统 在接下来的几年里，我们计划特别 检查氧自由基产生（和其他）的作用 潜在的介质）通过使用各种输注的 和雾化的淬灭剂和自由基清除剂， 对手。

Ozone and acid fog and acid rain (including sulfuric acid aerosols) are of major public health concern, in part because they are capable of producing measurable transient effects on the lungs of susceptible subjects, at concentrations which can occur in ambient pollutant episodes, and in part because they may act synergistically to produce effects on the lung greater than that produced by individual exposures. Knowledge concerning the possible deleterious effects of ozone-acid synargisms on the respiratory tract is still extremely meager. A sheep model system is proposed to examine the synergistic effect between ozone and respirable-sized sulfuric acid aerosols. In the current study we will use a multidisciplinary approach designed to evaluate the effects of ozone, acid aerosol, and their combination, on respiratory tract epithelial and endothelial permeability. Bronchoalveolar clearance of 99mTc-DTPA radioaersol from the lung will be used to determine epithelial permeability and results obtained will be compared to appearances of protein, lysosomal enzymes and cells in bronchoalveolar lavage carried out via a standard methodology. Sheep lung lymph will obtained to evaluate endothelial permeability and possible clearances of 99mTc-DTPA via lung lymphatics. Finally, in a separate series of sheep exposed to an identical protocol of ozone, acid aerosol or combined ozone-acid aerosol, airway blood flow will be continuously monitored using a Doppler flowmeter-telemetry system. Changes in airway blood flow are expected to provide an index of neurogenic or mediator- related events occurring in airway interstitium and which would be expected to effect airway smooth muscle as well as airway microvasculature. In subsequent years we plan to specifically examine for the role(s) of oxy radical productions (and other potential mediators) on this synergism via uses of various infused and aerosolized quenching agents and radical scavengers as in vivo antagonists.