OCULAR LENS STUDIES

这项工作描述在他的赠款是基于这样的前提下， 侮辱是成熟发展的起始或早期事件 发病性白内障 该赠款要求支持3项新举措， 在上述前提下。 (1)混合功能氧化反应的应用 用一种特殊的透镜蛋白，α晶体蛋白， 非二硫键和其他修饰，包括 B链磷酸丝氨酸位置附近。 工作 将导致澄清类似的修改期间出现的 发展为成熟期白内障。 (2)对影响的审查 氧化对透镜上皮细胞DNA的损伤、修复 和突变。 这项工作将试图探讨这样一个问题： 对DNA的氧化损伤可导致成熟期白内障。 (3)的 开发强大的生理相容性还原剂。 工作 将产生硫氧还蛋白肽类似物，理论上， 或修复对透镜的氧化损伤。 类似物将在 细胞和器官培养系统以确定它们是否有效。

The work described int his grant is based on the premise that oxidative insult is an initiating or early event in the development of maturity onset cataract. The grant requests support for 3 new initiatives based on the above premise. (1) The use of mixed function oxidation reactions with a specific lens protein, alpha crystallin, to elucidate covalent non-disulfide linkages and other modifications including alteration in the vicinity of the phosphoserine positions of the B chain. The work will lead to elucidation of similar modifications which arise during the development o maturity onset cataract. (2) An examination of the impact of oxidation on lens epithelial cell DNA with respect to damage, repair and mutation. The work will attempt to approach the question of whether oxidative damage to the DNA can lead to maturity onset cataract. (3) The development of powerful, physiologically compatible reductants. The work will produce thioredoxin peptide analogs which, in theory, should prevent or repair oxidative damage to the lens. The analogs will be tested in cell and organ culture systems to ascertain whether they are effective.