OCULAR FLUID COMPOSITION AND OCULAR TISSUE PHYSIOLOGY

提出了一个综合的研究方案来阐明相关的作用 发挥不同区域的血-眼屏障和不同的 正常眼内稳状态下眼内积液的分区 微环境；确定影响渗透性和 这些障碍物的传输特性；并定义改变的影响 阻隔功能对IOF组成和功能完整性的影响 眼内组织。在需要检验的假设中有以下几个： 玻璃体在维持眼部微环境中起着重要作用。 视网膜，尤指在急性缺血时，作为营养物质的储存库 作为代谢废物的水槽；以及急性或亚临床发作 眼部炎症或创伤会导致眼球累积恶化。 血-眼屏障功能。血眼通透性的研究 障碍将在体内进行研究。正常和更改的传输属性 (尤其是关于前列腺素运输)主要是组织的 与这些障碍相关的研究将在体内和体外进行。这个 将研究几种眼部炎症模型的时间进程，以确定 与每个炎症阶段相关联的单个介质，因此 这些介质对屏障功能和IOF组成的影响将是 探索过了。屏障功能恶化可能会导致 IOF中的痕量金属浓度异常，这反过来可能会导致 对不利的化学环境也将进行调查。临床部 我们提出的研究的意义随着越来越多的人 有证据表明，一些甚至大多数眼部疾病，尤其是缓慢的 发展为退行性疾病，如单纯性慢性青光眼、老花眼、 老年性白内障和黄斑变性是由老年性白内障和黄斑变性引起的 眼部微环境异常。

An integrated research program is proposed to elucidate the relative roles played by different regions of the blood-ocular barriers and by different compartments of intraocular fluid (IOF) in the homeostasis of normal ocular micro-environments; to identify the factors which affect the permeability and transport properties of these barriers; and to define the effects of altered barrier function on IOF composition and on the functional integrity of intraocular tissues. Among the hypotheses to be tested are the following: that the vitreous plays a major role in maintaing the micro-environment of the retina, especially during acute ischemia, by acting as a reservoir of nutrients and as a sink for metabolic wastes; and that episodes of acute or subclinical ocular inflammation or traumata contribute to the cumulative deterioration of blood-ocular barrier functions. Permeability properties of blood-ocular barriers will be studied in vivo. Normal and altered transport properties (especially with reference to prostaglandin transport) of the tissues primarily associated with these barriers will be studied both in vivo and vitro. The time-course of several models of ocular inflammation will be studied to identify the individual mediators associated with each inflammatory phase whereupon the effects of these mediators on barrier function and IOF composition will be explored. The possibility that deterioration of barrier functions may result in abnormal trace metal concentrations in the IOFs, which may, in turn, contribute to an adverse chemical milieu will also be investigated. The clinical significance of our proposed research becomes apparent in light of increasing evidence that some and possibly most ocular disorders, especially slowly developing degenerative disorders such as simple chronic glaucoma, presbyopia, senile cataract and macular degeneration are caused by or are associated with abnormalities in the ocular micro-environment.