PATHOGENESIS OF CORNEAL EDEMA AFTER INTRAOCULAR SURGERY

我们的总体目标是研究角膜水肿的发病机制 发生在眼内手术后。临床上，白内障后， 玻璃体切割术和眼前段手术，以及增加角膜厚度 血管内皮细胞就会丢失。厚度的增加要么是 基质水增加和/或基质丧失的结果 角膜缺失所致的蛋白多糖和糖蛋白 内皮屏障和/或泵功能。以下研究应包括 进一步加深对术后角膜水肿的认识和治疗。这个 这项建议的具体目的是：(1)确定角膜的作用 内皮细胞Na+/K+ATPase在角膜去肿中起重要作用。Na+/K+ATPase 通过哇巴因结合测量的密度将在兔、猫和 人，密度(泵功能)将与年龄相关， 再生和哇巴因抑制。(2)确定角膜的作用 内皮膜蛋白在维持内皮屏障中的作用 功能。主要的内皮膜蛋白将由 对照牛、兔和人的SDS-PAGE电泳法 血管内皮细胞和随后的屏障破坏。(3)将 角膜基质水肿性改变与基质丢失的关系 蛋白多糖、糖蛋白和胶原纤维的聚集。(4) 为了确定外科手术的效果(上皮清创术， 放射状角膜切开术、白内障手术)和药物(苯扎氯铵和 碳酸酐酶抑制剂)对角膜内皮屏障和泵的影响 功能。(5)测定基质中的可溶性蛋白质、蛋白多糖和 非肿胀弹性支角膜和非融合性角膜的糖蛋白 海洋硬骨鱼角膜基质。

Our overall objective is to study the pathogenesis of corneal edema which occurs following intraocular surgery. Clinically, following cataract, vitrectomy and anterior segment surgery, and increase in corneal thickness and a loss of endothelial cells occur. The increase in thickness is either the result of an increase in stromal water and/or the loss of stromal proteoglycans and glycoproteins resulting from a loss of the corneal endothelial barrier and/or pump function. The following studies should further our understanding and treatment of postsurgical corneal edema. The specific aims of this proposal are: (1) To determine what role corneal endothelial Na+/K+ ATPase has in corneal deturgescense. The Na+/K+ ATPase density as measured by ouabain binding will be studied in rabbit, cat and human, and the density (pump function) will be correlated to age, regeneration and ouabain inhibition. (2) To determine the role of corneal endothelial membrane proteins in the maintenance of the endothelial barrier function. The major endothelial membrane proteins will be determined by SDS-PAGE electrophoresis in bovine, rabbit and human in a controlled endothelium and following barrier disruption. (3) To correlate the morphology of the edematous corneal stroma to the loss of stromal proteoglycans, glycoproteins and the aggregation of collagen fibrils. (4) To determine the effects of surgical procedures (epithelial debridement, radial keratotomy, cataract surgery) and drugs (benzalkonium chloride and carbonic anhydrase inhibitors) on the corneal endothelial barrier and pump function. (5) To measure the stromal soluble proteins, proteoglycans and glycoproteins of the non-swelling elasmobranch cornea and the non-fused stroma of the marine teleost cornea.