OCULAR LENS STUDIES

在他的资助中描述的工作是基于氧化的前提 侮辱是成熟发展过程中的初始事件或早期事件 发作性白内障。赠款要求支持3项新的计划，这些计划基于 在上述前提下。(1)混合功能氧化反应的使用 用一种特定的晶状体蛋白，α晶体蛋白来阐明共价 非二硫键和其他修饰，包括改变 B链的磷酸丝氨酸位置附近。这项工作 将导致对类似修改的澄清 成熟期发作性白内障的发生。(二)审查影响 氧化对晶状体上皮细胞DNA的损伤、修复 和突变。这项工作将试图探讨这样一个问题： DNA的氧化损伤会导致成熟期白内障。(3) 开发强大的、生理上相容的还原剂。这项工作 会产生硫氧还蛋白多肽类似物，从理论上讲，这应该会阻止 或者修复晶状体的氧化损伤。这些类比将在 细胞和器官培养系统以确定它们是否有效。

The work described int his grant is based on the premise that oxidative insult is an initiating or early event in the development of maturity onset cataract. The grant requests support for 3 new initiatives based on the above premise. (1) The use of mixed function oxidation reactions with a specific lens protein, alpha crystallin, to elucidate covalent non-disulfide linkages and other modifications including alteration in the vicinity of the phosphoserine positions of the B chain. The work will lead to elucidation of similar modifications which arise during the development o maturity onset cataract. (2) An examination of the impact of oxidation on lens epithelial cell DNA with respect to damage, repair and mutation. The work will attempt to approach the question of whether oxidative damage to the DNA can lead to maturity onset cataract. (3) The development of powerful, physiologically compatible reductants. The work will produce thioredoxin peptide analogs which, in theory, should prevent or repair oxidative damage to the lens. The analogs will be tested in cell and organ culture systems to ascertain whether they are effective.