RETINAL NEUROTRANSMITTERS AND SYNAPTIC CIRCUITRY

这项研究的目标是确定神经化学物质是如何 猫视网膜回路在其加工过程中的作用 视觉信息。具体地说，它试图确定 形态定义神经元的神经递质含量 亚群；阐明突触之间的关系 具有化学特性的细胞；确定传递体的变化 在不同刺激条件下的水平；并确定 神经化学输入以确定神经节细胞群。这个 这些发现将与正在进行的关于 这些神经元的形态、生理学和药理学。一个 了解特定化学成分的神经元如何在大脑的 视网膜不仅将对模型的开发做出贡献 视网膜功能，也将有助于了解 递质通过神经系统发挥作用。 将使用高尔基和放射自显影相结合的技术。 以观察积累甘氨酸、GABA和牛磺酸的细胞。 使用这种技术的量化将被用来研究 特定神经元对一种假定递质的亲和力 不同的生理条件。 将产生针对甘氨酸、GABA、牛磺酸、 谷氨酸和天冬氨酸，用于免疫细胞化学 学习。每个抗血清将针对一种氨基酸。 它与牛血清白蛋白结合。法布 抗血清的片段将被制备并用于电子 显微镜。高尔基体结合免疫细胞化学研究 将被用来鉴定特定的化学细胞并确定 它们与其他神经元的关系。双重标签研究将 被用来定义发射机协同定位和 阐明化学特定细胞之间的突触关系。

The goal of this study is to determine how the neurochemical circuitry of cat retina contributes to its function in processing visual information. Specifically, it seeks to determine the neurotransmitter content of morphologically defined neuronal subpopulations; to elucidate the synaptic relationships between chemically specific cells; to determine changes in transmitters levels under various stimulus conditions; and to ascertainthe neurochemical input to define populations of ganglion cell. The findings will be correlated with on-going studies of the morphology, physiology, and pharmacology of these neurons. A knowledge of how chemically specific neurons interact within the retina will contribute not only to the development of models of retinal function but will also assist in the understanding of transmitter function throughotu the nervous system. Combined Golgi and autoradiographic techniques will be employed to visualize cells which accumulate glycine, GABA, and taurine. Quantitation with this technique will be used to investigate the affinity of specific neurons for a putative transmitter under varying physiological conditions. Antisera will be generated against glycine, GABA, taurine, glutamate, and aspartate and used in immunocytochemical studies. Each antiserum will be directed against an amino acid which ahs been conjugated to bovine serum albumin. Fab fragments of the antisera will be prepared and used for electron microscopy. Combined Golgi and immunocytochemical studies will be used to identify chemically specific cells and to determine their relationships with other neurons. Double label studies will be employed to define transmitter co-localizations and to elucidate synaptic relationships between chemically specific cells.