DOES EXPOSURE OF HUMAN CELLS TO EM FIELDS CAUSE CANCER

这项研究将调查暴露在细胞之间的拟议联系 到极低频(ELF)电磁场和 恶毒。我们将确定肿瘤的特征 暴露在空气中的人细胞发生转化和/或永生化 表征了60赫兹的ELF电磁场。目标1.确定是否 暴露于人的细胞中原癌基因的表达发生改变 通过测量mRNA转录本和结果的水平来实现ELF电磁场 蛋白质。最初将使用对已定义的ELF电磁场的短期曝光； 进一步的实验将确定时间进程或其他参数如何 暴露的程度会影响信使核糖核酸水平。目的2.确定细胞是否分裂 频率随电磁场暴露的变化而变化。目的3.调查 细胞对极低频电磁场响应的信号转导；可以与 进行信号转导。与之前的研究预测的一样？ 我们的主要目标是获得足够的背景信息，以最终 允许我们试验性地测试ELF电磁场暴露是否可以起到 在引发或增强肿瘤疾病方面的作用。其后果是 然而，这项研究的结果与暴露的总体安全性有关。 到工业和日常生活中的电磁领域。

This research will investigate the proposed link between exposure of cells to extremely low frequency (ELF) electromagnetic (EM) fields and malignancy. We will determine if characteristics of neoplastic transformation and/or immortalization occur in human cells exposed to characterized ELF EM fields at 60 Hz. Aim 1. To determine if the expression of cellular proto-oncogenes is altered in human cells exposed to ELF EM fields by measurement of levels of mRNA transcripts and resulting proteins. Short exposures to defined ELF EM fields will be used initially; further experiments will determine how the time course or other parameters of exposure affect mRNA levels. Aim 2. To determine if cell divisional rates are changed by EM field exposure. Aim 3. To investigate the role of signal transduction in cell response to ELF EM fields; can a relation with signal transduction be made. as predicted on the basis of previous studies? Our major aim is to obtain adequate background information to ultimately allow us to experimentally test whether ELF EM field exposure can play a role in initiating, or augmenting neoplastic disease. The ramifications of this research, however, have bearing on the general safety of exposure to EM fields in industrial and everyday life.