ACTIVATION OF THE HEXOSEMONOHOSPHATE SHUNT IN THE LENS

磷酸己糖分流及其相关的研究 在本申请中提出了途径。 我们将检查以下内容 五个主题：1）HMPS的控制; 2）HMPS的活动及其 相关途径; 3）重新检查多元醇途径和以前的 未识别的限速步骤; 4）干扰的意义， 膜代谢;和5）氧化对基础HMPS活性的影响 和抗氧化性。 这些研究是基于过去两年获得的数据。 我们有 发现证据：1）醛糖还原酶和 谷胱甘肽还原酶和2）膜代谢物的显着变化， 糖尿病大鼠 我们将采用生化程序， 生物物理方法，如核磁共振光谱， 检查各种NADPH需要途径和电子 顺磁共振光谱法来探测膜结构变化。 这些研究对于了解糖尿病的发病机制至关重要。 白内障形成 抗氧化性的研究可以为 为设计非手术抗老年性白内障治疗提供了依据。

Studies on the hexose monophosphate shunt (HMPS) and its associated pathways are proposed in this application. We will examine the following five topics: 1) Control of the HMPS; 2) Activity of the HMPS and its associated pathways; 3) Re-examination of the polyol pathway and previously unrecognized rate-limiting steps; 4) The significance of disturbance to membrane metabolism; and 5) The effect of oxidation on basal HMPS activity and oxidative resistance. These studies are based on data obtained over the past two years. We have found evidence of: 1) competition for NADPH between aldose reductase and glutathione reductase and 2) prominent changes in membrane metabolites in the diabetic rats. We will employ biochemical procedures as well as biophysical methods such as nuclear magnetic resonance spectroscopy to examine the interaction of various NADPH-requiring pathways and electron paramagnetic resonance spectroscopy to probe membrane structural change. These studies are crucial to the understanding of diabetic cataractogenesis. Studies on oxidative resistance can pave the groundwork for the design of non-surgical anti-senile cataract therapy.