ELECTROPHYSIOLOGY OF CILIARY BODY TRANSPORT PROCESSES

这项建议的长期目标是调查 与房水的产生有关的细胞机制 纤毛上皮。具体目标是体外电生理学 纤毛分离制剂的转运过程研究 上皮组织。这些实验将提供有关正常的 涉及的生理机制和药物诱导的改变 在积极产生房水的过程中。房水复位术 通过药物治疗形成是一种常见的治疗隆起的方法 与青光眼相关的眼压。关于以下方面的知识 负责房水产生的细胞机制将提供 对房水形成的正常速率和可能的洞察力 房水发生率降低的药理学机制 制作。 分离的虹膜-睫状体制剂安装在小室中 用于测量跨上皮电参数(电位 差值、短路电流、组织电阻)、净液 迁移和单向通量(离子、水、非电解质)。 这些研究代表了主动运输和流体的巩固 保持正常解剖结构的组织制剂中的流动 睫状体双上皮层之间的关系 流程。常规微管穿刺术的研究 膜电位将与 跨上皮测量。这些研究将提供关于 单个睫状体上皮细胞与离子运动和 运输系统。电生理检查将用于 研究以下几类药物：(一)卤化吸入 麻醉剂，(Ii)肾上腺素能激动剂(IIa) 神经肽Y与肾上腺素能激动剂，(Iii)巴比妥酸盐，和(Iv) 叠氮化钠。这些实验将提供有关机理的信息。 参与房水的主动产生，并可能导致新的 和独特的抗青光眼药物设计来抑制不同的 房水产生的顺序步骤。

The long-term objective of this proposal is the investigation of cellular mechanisms relating to the production of aqueous humor by the ciliary epithelium. Specific aims are the in vitro electrophysiologic study of transport processes on isolated preparations of the ciliary epithelium. These experiments will furnish information on normal physiological mechanisms and pharmacological-induced alterations involved in the active production of aqueous humor. Reduction of aqueous humor formation by medical therapy is a frequent treatment of the elevated intraocular pressure associated with glaucoma. Knowledge regarding cellular mechanisms responsible for aqueous humor production will provide insights into the normal rate of aqueous humor formation and possible pharmacologic mechanisms for the reduction in the rate of aqueous humor production. Isolated iris-ciliary body preparations are mounted in chambers for measurement of transepithelial electrical parameters (potential difference, short-circuit current, tissue resistance), net fluid transport, and unidirectional fluxes (ionic, water, non-electrolyte). These studies represent a consolidation of active transport and fluid flow in a tissue preparation maintaining the normal anatomic relationships between the double epithelial layers of the ciliary processes. Conventional micropipet impalement studies for measurement of membrane potential will be performed in conjunction with the transepithelial measurements. These studies will provide information on individual ciliary epithelial cells in relationship to ion movements and transport systems. Electrophysiologic investigations will be used to study the following classes of drugs: (i) halogenated inhalation anesthetic agents, (ii) adrenergic agonists (iia) the interaction of neuropeptide Y with the adrenergic agonist, (iii) barbiturates, and (iv) sodium azide. These experiments will furnish information on mechanisms involved in the active production of aqueous humor and may result in new and unique antiglaucoma agents designed to inhibit the different sequential steps in aqueous humor production.